肾脏中核糖体前体核糖核酸的代谢
Metabolism of ribosomal precursor ribonucleic acid in kidney.
作者信息
Ab G, Malt R A
出版信息
J Cell Biol. 1970 Aug;46(2):362-9. doi: 10.1083/jcb.46.2.362.
Abstract
The labile precursors of ribosomal RNA in mouse kidney are preserved when nuclei rapidly isolated after sieving through multiple screens are swollen and cleansed in the presence of an RNase inhibitor before digestion with DNase and phenol extraction. The kinetics of nucleolar labeling analyzed on polyacrylamide gels show that 36S RNA is the major intermediate product in the catabolism of the original 45S RNA precursor to 32S RNA, from which 28S RNA is derived. Each kidney nucleus contains about 200-600 molecules of 45S RNA; the turnover time of the 45S pool is about 3 +/- 2 min. Compared with HeLa cells, kidney nuclei have a different major intermediate product and a much smaller and more rapidly turning-over pool of ribosomal precursor RNA.
摘要
当通过多层筛网快速分离出的小鼠肾脏细胞核在核糖核酸酶抑制剂存在的情况下肿胀并清洗后，再用脱氧核糖核酸酶消化并进行酚抽提时，核糖体核糖核酸的不稳定前体得以保留。在聚丙烯酰胺凝胶上分析核仁标记的动力学表明，36S核糖核酸是原始45S核糖核酸前体分解代谢为32S核糖核酸的主要中间产物，而28S核糖核酸则来源于32S核糖核酸。每个肾脏细胞核含有约200 - 600个45S核糖核酸分子；45S核糖核酸库的周转时间约为3±2分钟。与海拉细胞相比，肾脏细胞核具有不同的主要中间产物，并且核糖体前体核糖核酸库要小得多且周转更快。
相似文献
1
Metabolism of ribosomal precursor ribonucleic acid in kidney.肾脏中核糖体前体核糖核酸的代谢
J Cell Biol. 1970 Aug;46(2):362-9. doi: 10.1083/jcb.46.2.362.
2
Ribonucleic acid synthesis in cells infected with herpes simplex virus. I. Patterns of ribonucleic acid synthesis in productively infected cells.感染单纯疱疹病毒的细胞中的核糖核酸合成。I. 生产性感染细胞中核糖核酸合成的模式
J Virol. 1969 Jul;4(1):36-46. doi: 10.1128/JVI.4.1.36-46.1969.
3
Synthesis and maturation of ribosomal ribonucleic acids in isolated HeLa cell nuclei. A tracer study on the topology of the 45S precursor of ribosomal ribonucleic acids.离体海拉细胞核中核糖体核糖核酸的合成与成熟。核糖体核糖核酸45S前体拓扑结构的示踪研究。
Biochem J. 1974 Aug;142(2):263-72. doi: 10.1042/bj1420263.
4
Nucleolar ribosomal precursor RNA and protein metabolism in human embryo kidney cultures infected with adenovirus 12.感染腺病毒12的人胚肾培养物中的核仁核糖体前体RNA和蛋白质代谢
Virology. 1972 Jul;49(1):79-89. doi: 10.1016/s0042-6822(72)80008-4.
5
RNA metabolism in HeLa cells at reduced temperature. I. Modified processing of 45S RNA.低温下HeLa细胞中的RNA代谢。I. 45S RNA的加工修饰
J Cell Biol. 1971 Sep;50(3):818-29. doi: 10.1083/jcb.50.3.818.
6
Intranuclear maturation pathways of rat liver ribosomal ribonucleic acids.大鼠肝脏核糖体核糖核酸的核内成熟途径
Biochem J. 1976 Dec 15;160(3):495-503. doi: 10.1042/bj1600495.
7
The selective interruption of nucleolar RNA synthesis in HeLa cells by cordycepin.虫草素对HeLa细胞中核仁RNA合成的选择性阻断
J Cell Biol. 1969 May;41(2):510-20. doi: 10.1083/jcb.41.2.510.
8
Ribosomal RNA synthesis and processing in a particulate site in the HeLa cell nucleus.核糖体RNA在海拉细胞核内一个颗粒状位点的合成与加工
Science. 1966 Nov 11;154(3750):786-9. doi: 10.1126/science.154.3750.786.
9
Evidence for the existence of several molecular species in the "45S fraction" of mammalian ribosomal precursor RNA.哺乳动物核糖体前体RNA“45S组分”中存在多种分子种类的证据。
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1117-20. doi: 10.1073/pnas.68.6.1117.
10
Metabolic studies of small molecular weight nuclear RNA components in BHK-21 cells.BHK - 21细胞中小分子量核RNA组分的代谢研究。
Biochim Biophys Acta. 1974 Feb 27;340(1):64-76. doi: 10.1016/0005-2787(74)90174-9.
引用本文的文献
1
Hidden breaks in ribosomal RNA of phylogenetically tetraploid fish and their possible role in the diploidization process.系统发育四倍体鱼类核糖体RNA中的隐藏断裂及其在二倍体化过程中的可能作用。
Biochem Genet. 1983 Aug;21(7-8):819-41. doi: 10.1007/BF00498929.
2
Kinetics of RNA synthesis in toad bladder epithelium: action of aldosterone during the latent period.蟾蜍膀胱上皮细胞中RNA合成的动力学：潜伏期醛固酮的作用
J Clin Invest. 1971 Mar;50(3):543-51. doi: 10.1172/JCI106523.
3
The oestrogen-stimulated synthesis of heterogeneous nuclear ribonucleic acid in the uterus of immature rats.雌激素刺激未成熟大鼠子宫中异质性核核糖核酸的合成。
Biochem J. 1973 Apr;131(4):689-97. doi: 10.1042/bj1310689.
4
Ribonucleic acid labelling and nucleotide pools during compensatory renal hypertrophy.代偿性肾肥大过程中的核糖核酸标记与核苷酸库
Biochem J. 1974 Dec;144(3):447-53. doi: 10.1042/bj1440447.
5
Conservation of ribosomal RNA during compensatory renal hypertrophy. A major mechanism in RNA accretion.代偿性肾肥大过程中核糖体RNA的保守性。RNA积聚的一种主要机制。
J Cell Biol. 1976 Jun;69(3):548-56. doi: 10.1083/jcb.69.3.548.
6
Ribosomal RNA metabolism during renal hypertrophy. Evidence of decreased degradation of newly synthesized ribosomal RNA.肾脏肥大过程中的核糖体RNA代谢。新合成的核糖体RNA降解减少的证据。
J Cell Biol. 1975 Jan;64(1):260-5. doi: 10.1083/jcb.64.1.260.
7
Short-lived methylated messenger RNA in mouse kidney.小鼠肾脏中的短寿命甲基化信使核糖核酸
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2609-13. doi: 10.1073/pnas.73.8.2609.
8
Macromolecular metabolism in compensatory renal hypertrophy.代偿性肾肥大中的大分子代谢
Yale J Biol Med. 1978 May-Jun;51(3):419-28.
本文引用的文献
1
The desoxyribonucleic acid content of animal cells and its evolutionary significance.动物细胞的脱氧核糖核酸含量及其进化意义。
J Gen Physiol. 1951 Mar 20;34(4):451-62. doi: 10.1085/jgp.34.4.451.
2
Sedimentation characteristics of rapidly labelled RNA from HeLa cells.来自海拉细胞的快速标记RNA的沉降特性。
Biochem Biophys Res Commun. 1962 Jun 4;7:486-90. doi: 10.1016/0006-291x(62)90341-8.
3
ENTRANCE OF NEWLY FORMED MESSENGER RNA AND RIBOSOMES INTO HELA CELL CYTOPLASM.新形成的信使核糖核酸和核糖体进入海拉细胞细胞质。
J Mol Biol. 1965 Feb;11:187-201. doi: 10.1016/s0022-2836(65)80050-x.
4
ROLE OF THE NUCLEOLUS IN RIBONUCLEIC ACID METABOLISM AND OTHER CELLULAR PROCESSES.核仁在核糖核酸代谢及其他细胞过程中的作用。
Natl Cancer Inst Monogr. 1964 May;14:73-89.
5
Isolation of pure and unaltered liver nuclei morphology and biochemical composition.分离出形态和生化组成纯净且未改变的肝细胞核。
Exp Cell Res. 1956 Aug;11(2):317-21. doi: 10.1016/0014-4827(56)90107-0.
6
Methylation of 45 s ribosomal RNA precursor in HeLa cells.HeLa细胞中45s核糖体RNA前体的甲基化
J Mol Biol. 1967 Jan 28;23(2):149-61. doi: 10.1016/s0022-2836(67)80023-8.
7
Rapidly labeled HeLa cell nuclear RNA. I. Identification by zone sedimentation of a heterogeneous fraction separate from ribosomal precursor RNA.快速标记的海拉细胞核RNA。I. 通过区带沉降法鉴定与核糖体前体RNA分离的异质组分。
J Mol Biol. 1966 Aug;19(2):349-61. doi: 10.1016/s0022-2836(66)80009-8.
8
RNA metabolism in the HeLa cell nucleus.海拉细胞核中的RNA代谢
J Mol Biol. 1966 May;17(1):117-30. doi: 10.1016/s0022-2836(66)80098-0.
9
The subunits and structural ribonucleic acids of Jensen sarcoma ribosomes.詹森肉瘤核糖体的亚基和结构核糖核酸。
Biochim Biophys Acta. 1966 Feb 21;114(2):264-76. doi: 10.1016/0005-2787(66)90308-x.
10
Ribosomal RNA synthesis and processing in a particulate site in the HeLa cell nucleus.核糖体RNA在海拉细胞核内一个颗粒状位点的合成与加工
Science. 1966 Nov 11;154(3750):786-9. doi: 10.1126/science.154.3750.786.
Zotero 插件