Penman S, Smith I, Holtzman E
Science. 1966 Nov 11;154(3750):786-9. doi: 10.1126/science.154.3750.786.
A particulate fraction has been isolated from detergent-prepared HeLa cell nuclei. The fraction consists largely of organelles that resemble the nucleoli of intact cells. The 45S RNA that is precursor to 28S and 18S ribosomal RNA is associated with the fraction. The 32S RNA that is labeled after the 45S RNA and is the apparent precursor to 28S RNA is also associated with the fraction. The nucleoplasm contains 28S RNA that behaves as an intermediate between the 32S nucleolar RNA and the 28S cytoplasmic RNA.
已从用去污剂处理过的海拉细胞核中分离出一种颗粒成分。该成分主要由类似于完整细胞中核仁的细胞器组成。作为28S和18S核糖体RNA前体的45S RNA与该成分相关联。在45S RNA之后被标记且明显是28S RNA前体的32S RNA也与该成分相关联。核质中含有28S RNA，其表现为32S核仁RNA和28S细胞质RNA之间的中间体。
