Talmadge J E, Starkey J R, Davis W C, Cohen A L
J Supramol Struct. 1979;12(2):227-43. doi: 10.1002/jss.400120208.
An experimental system for the study of metastasis has been developed using an epithelioid cell line of hepatic origin which had previously been chemically transformed in vitro. These metastatic cells were studied in the syngeneic rat strain. The cloned parent cell line metastasizes only to the lungs following intravenous, subcutaneous, or intraperitoneal injection. The metastatic phenotype is stable during in vitro passage, and subclones from the parent clone have a metastatic capacity statistically similar to that of the parent clone. Following ascites passage of the parent cell line, the cell population obtained exhibits the same metastatic ability as the parent clone. However, subclones obtained from the ascites-passaged population exhibit metastatic heterogeneity. This heterogeneity is introduced by the host passage and not by in vitro culture or subcloning. In the case of the two metastatic variants examined, the difference in the metastatic phenotype is found not to be due to differences in arrest or trapping of the cells but appears to be related to long-term survival and proliferation of the tumor cells following their arrest in the lungs. Morphologically the variants are very similar, and growth of the metastatic foci provokes a vigorous inflammatory response by the host.
利用一种源自肝脏的上皮样细胞系开发了一种用于研究转移的实验系统，该细胞系先前已在体外进行化学转化。在同基因大鼠品系中对这些转移细胞进行了研究。克隆的亲本细胞系在静脉内、皮下或腹腔注射后仅转移至肺部。转移表型在体外传代过程中是稳定的，亲本克隆的亚克隆具有与亲本克隆在统计学上相似的转移能力。亲本细胞系经腹水传代后，获得的细胞群体表现出与亲本克隆相同的转移能力。然而，从经腹水传代的群体中获得的亚克隆表现出转移异质性。这种异质性是由宿主传代引入的，而非体外培养或亚克隆。在所检测的两个转移变体的情况下，转移表型的差异并非由于细胞的滞留或捕获差异所致，而是似乎与肿瘤细胞在肺部滞留后的长期存活和增殖有关。从形态学上看，这些变体非常相似，转移灶的生长引发宿主强烈的炎症反应。
