Starkey J R, Davis W C, Talmadge J E
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164.
Cancer Immunol Immunother. 1982;14(2):124-31. doi: 10.1007/BF00200180.
The immunological characteristics of two series of metastatic variants of restricted genetic origin were related to their lung-colony-forming potential. A series of metastatic variants was isolated from a tumor-cell population in which heterogeneity appeared following short-term in vivo passage, while a second series of variants were immunoselected in vitro for resistance to antibody-complement-mediated cell lysis. In the case of the first series, the sensitivity of the individual variants to cell-mediated and antibody-complement-mediated cytotoxicity appeared to be partly determined by the number and rate of loss of antibody-binding sites on the cell surface. These characteristics also correlated with the efficiency of experimental metastasis in the animal. We compared the results reported herein with our previous studies of nonimmune parameters for this series of metastatic variants, and we conclude that immunological differences can be important to the efficiency of lung-colony formation. However, in the case of the second series of variants, despite selection in vitro for resistance to antibody-complement-mediated cell lysis, the behavior of these variants in the lung colony assay could not be predicted by the immunologic parameters examined.
两个具有特定遗传起源的转移变体系列的免疫特性与其肺集落形成潜力相关。从一个肿瘤细胞群体中分离出一系列转移变体，该群体在短期体内传代后出现异质性，而另一系列变体则在体外进行免疫选择以抵抗抗体-补体介导的细胞裂解。对于第一系列，各个变体对细胞介导和抗体-补体介导的细胞毒性的敏感性似乎部分取决于细胞表面抗体结合位点的数量和丧失速率。这些特性也与动物实验性转移的效率相关。我们将本文报道的结果与我们先前对该系列转移变体的非免疫参数研究进行了比较，得出结论：免疫差异对肺集落形成效率可能很重要。然而，对于第二系列变体，尽管在体外选择了对抗体-补体介导的细胞裂解具有抗性，但这些变体在肺集落试验中的行为无法通过所检测的免疫参数来预测。
