Labeyrie E, Koechlin Y
J Neurosci Methods. 1979 Mar;1(1):35-9. doi: 10.1016/0165-0270(79)90005-0.
In order to study calcium transients in the central nervous system of vertebrates a microphotoelectrode was designed. It is composed of three channels, through the first aequorin is perfused into the extracellular space under constant pressure, the second is an optical glass light-conducting channel, and the third a classical NaCl-filled recording pipette. The light emitted by the interaction of the injected aequorin with the local extracellular calcium is transmitted through the optic fiber to a photomultiplier of high sensitivity.
为了研究脊椎动物中枢神经系统中的钙瞬变，设计了一种微光电极。它由三个通道组成，通过第一个通道，水母发光蛋白在恒压下灌注到细胞外空间，第二个通道是光学玻璃导光通道，第三个通道是传统的充满氯化钠的记录微电极。注入的水母发光蛋白与局部细胞外钙相互作用发出的光通过光纤传输到高灵敏度的光电倍增管。
