通过蔗糖溶液沉降对大鼠肝脏微粒体蛋白质合成能力的影响。
Effect of sedimentation through sucrose solutions on the protein-synthesizing ability of rat liver microsomes.
作者信息
Leslie R A, Mansbridge J N
出版信息
Biochem J. 1970 May;117(5):893-8. doi: 10.1042/bj1170893.
Abstract
- Centrifugation of the postmitochondrial supernatant of rat liver through 1.0m-sucrose produces particles that have 85-95% less incorporating ability in a cell-free protein-synthesizing system than either ribosomes or microsomes. 2. The incorporation of [(14)C]phenylalanine into protein by particles prepared by sedimentation through 1.0m-sucrose is stimulated about 20-fold by addition of poly U. 3. The content of rapidly labelled RNA of microsomes is decreased during centrifugation through 1.0m-sucrose. 4. It is suggested that degradation of mRNA occurs during the formation of the pellet in the centrifuge tube as a result of a membrane-bound alkaline ribonuclease, after removal of the ribonuclease inhibitor of the soluble fraction.
摘要
- 大鼠肝脏线粒体后上清液经1.0m蔗糖离心后产生的颗粒，在无细胞蛋白质合成系统中的掺入能力比核糖体或微粒体低85 - 95%。2. 通过1.0m蔗糖沉降制备的颗粒将[（14）C]苯丙氨酸掺入蛋白质的过程，因加入多聚U而受到约20倍的刺激。3. 微粒体快速标记RNA的含量在经1.0m蔗糖离心过程中减少。4. 有人提出，在去除可溶性部分的核糖核酸酶抑制剂后，由于一种膜结合碱性核糖核酸酶的作用，在离心管沉淀形成过程中发生了mRNA的降解。
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