Tsonev Ts S, Karaivanov L, Shishkov N, Milanov M L, Chilev D
Vet Med Nauki. 1979;16(5):59-66.
Atypically developing chicken septicemia was studied at 3 farms in Bulgaria. In one of the observed centers of chicken septicemia infection no pathologic anatomical changes were evident while in the other two centers of infection serofibrinose and serofibrinose vitelline peritonitis, as well as pleurisy were present. In the bacteriological investigation carried out on 2969 samples 233 strains of chicken septicemia were isolated. Identification of 84 of them by the aid of the specific bacteriophage P. multocida 115 and of sugar and alcohol fermantation revealed that they can be classified as P. multocida. They are lyzated by a phage but do not reproduce it. Chicken septicemia cultures are virulent for white mice. In intramuscular injections of pullets these cultures prove not virulent, but their intravenous application kills the pullets in 5--10 days and the initial strain is isolated from all inner organs. The biological test on pullets is suitable for determining chicken septicemia culture virulence.
在保加利亚的3个农场对非典型发展的鸡败血症进行了研究。在观察到的鸡败血症感染中心之一,没有明显的病理解剖变化,而在其他两个感染中心,存在浆液纤维素性和浆液纤维素性卵黄性腹膜炎以及胸膜炎。在对2969份样本进行的细菌学调查中,分离出233株鸡败血症菌株。借助特异性多杀性巴氏杆菌噬菌体115以及糖和酒精发酵对其中84株进行鉴定,结果表明它们可归类为多杀性巴氏杆菌。它们被噬菌体裂解,但不产生噬菌体。鸡败血症培养物对小白鼠具有毒性。对小母鸡进行肌肉注射时,这些培养物证明无毒,但静脉注射则会在5至10天内杀死小母鸡,并且从所有内部器官中都能分离出原始菌株。对小母鸡进行的生物学试验适用于确定鸡败血症培养物的毒性。