Korudzhiĭski N
Vet Med Nauki. 1979;16(9):33-41.
Hundred twenty three samples of bull semen fluid frozen at 196 degrees C including 83 plastic ampules, 20 granules and 20 plastic straws obtained from the containers of the insemination stations of 10 farms from the Sofia district were investigated. Two hundred twelve strains were isolated and identified as: Escherichia coli--25 strains, Hafnia--16 strains, Citrobacter, Enterobacter and Proteus mirabilis--9 strains of each. The remaining Gram-negative genera and species were more rarely encountered. Gram positive bacteria: Micrococcus--19 strains, Staphylococcus aureus--17 strains, Staph. epidermidis--15 strains, Bacillus cereus--15 strains, B. subtilis--12 strains. Other representatives of Gram-positive bacteria were also found but in lower percentages. Least bacteria were observed in semen fluid frozen in plastic straws and most--in plastic ampules which were mainly used until recently for cow insemination. It was established that the same bacteria isolated by other authors from fresh sperm were encountered in semen fluid stored at minus temperatures. The conclusion is made that semen fluid stored at low temperature is contaminated with bacteria. It is only natural that these bacteria are introduced in cow genitals by insemination.
对来自索非亚地区10个农场输精站容器中的123份冷冻于196摄氏度的公牛精液样本进行了调查，其中包括83个塑料安瓿、20个颗粒和20个塑料细管。共分离并鉴定出212株菌株，分别为：大肠杆菌——25株，哈夫尼亚菌——16株，柠檬酸杆菌、肠杆菌和奇异变形杆菌——各9株。其余革兰氏阴性菌属和菌种较少见。革兰氏阳性菌：微球菌——19株，金黄色葡萄球菌——17株，表皮葡萄球菌——15株，蜡样芽孢杆菌——15株，枯草芽孢杆菌——12株。也发现了其他革兰氏阳性菌代表，但比例较低。在塑料细管中冷冻的精液中观察到的细菌最少，而在主要用于奶牛输精的塑料安瓿中最多。已确定其他作者从新鲜精子中分离出的相同细菌也存在于低温保存的精液中。得出的结论是，低温保存的精液被细菌污染。通过输精将这些细菌引入奶牛生殖道是很自然的。
