兔乳腺中依赖DNA的RNA聚合酶的分离与鉴定
Isolation and characterization of DNA-dependent RNA polymerases from rabbit mammary gland.
作者信息
Kleczkowska D, Chomczyński P
出版信息
Acta Biochim Pol. 1979;26(4):349-57.
PMID:545958
Abstract
RNA polymerases AI, AII, BI, BII, CI and CII were found in the mammary gland from lactating rabbits. The enzymes obtained from total cell homogenates were partially purified and separated by DEAE-Sephadex chromatography. Their chromatographic properties, alpha-amanitin-sensitivity, template specificity, ionic strength and divalent cation requirements are described.
摘要
在泌乳期兔子的乳腺中发现了RNA聚合酶AI、AII、BI、BII、CI和CII。从全细胞匀浆中获得的这些酶经过部分纯化，并通过DEAE-葡聚糖凝胶色谱法进行分离。文中描述了它们的色谱特性、α-鹅膏蕈碱敏感性、模板特异性、离子强度和二价阳离子需求。
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