Grade K, Schönheit C, Lindigkeit R
Acta Biol Med Ger. 1977;36(1):29-34.
A simplified method is described for the large-scale preparation of a highly purified DNA-dependent RNA polymerase B from calf thymus. The method includes homogenization and lysis of the tissue, chromatography on DEAE-cellulose, phosphocellulose, hydroxylapatite-Sephadex G-10 and, once again, phosphocellulose. The procedure avoids the preparation of nuclei, the use of sonication, ammonium sulphate precipitations and dialysis steps and needs no ultracentrifugation.
本文描述了一种从牛胸腺中大规模制备高纯度DNA依赖性RNA聚合酶B的简化方法。该方法包括组织的匀浆和裂解、在DEAE-纤维素、磷酸纤维素、羟基磷灰石-葡聚糖凝胶G-10上进行层析，以及再次在磷酸纤维素上进行层析。该程序避免了细胞核的制备、超声处理、硫酸铵沉淀和透析步骤，并且不需要超速离心。
