关于海胆受精膜晶体成分的重构。
On the reconstitution of the crystalline components of the sea urchin fertilization membrane.
作者信息
Bryan J
出版信息
J Cell Biol. 1970 Jun;45(3):606-14. doi: 10.1083/jcb.45.3.606.
Abstract
The characteristics of the reconstitution of a crystalline component of the sea urchin fertilization membrane are presented. The reassembly of large aggregates of cylindrical or tubular components is effected by the addition of calcium or other divalent cations. The reassembly requires a slightly alkaline pH and is little affected by increasing ionic strength. Reassembly is strongly inhibited by treatment with reducing agents such as dithiothreitol. The role of this protein in the formation of the fertilization membrane and its possible relation to the calcium-insoluble proteins of the mitotic apparatus are discussed.
摘要
本文介绍了海胆受精膜晶体成分重构的特征。通过添加钙或其他二价阳离子，可实现圆柱形或管状成分的大聚集体的重新组装。重新组装需要略呈碱性的pH值，且受离子强度增加的影响较小。用二硫苏糖醇等还原剂处理会强烈抑制重新组装。本文还讨论了这种蛋白质在受精膜形成中的作用及其与有丝分裂器中钙不溶性蛋白质的可能关系。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Electron microscope study of mitosis in sea urchin blastomeres.海胆卵裂球有丝分裂的电子显微镜研究。
J Biophys Biochem Cytol. 1961 Nov;11(2):419-31. doi: 10.1083/jcb.11.2.419.
3
Changes in the cortical layer of sea urchin eggs at fertilization as studied with the electron microscope. I. Clypeaster japonicus.用电镜研究海胆卵受精时皮层的变化。I. 日本笠海胆
Exp Cell Res. 1961 Nov;25:383-97. doi: 10.1016/0014-4827(61)90288-9.
4
An electron microscope study of fertilisation of the sea urchin egg Psammechinus miliaris.对海胆卵（粒刺海胆）受精过程的电子显微镜研究。
Exp Cell Res. 1961 Jan;22:45-55. doi: 10.1016/0014-4827(61)90085-4.
5
Acid and base production at fertilization in the sea urchin.海胆受精过程中的酸碱产生
Exp Cell Res. 1961 Jan;22:233-45. doi: 10.1016/0014-4827(61)90101-x.
6
The direct isolation of the mitotic apparatus.有丝分裂器的直接分离。
J Biophys Biochem Cytol. 1961 Aug;10(4):467-74. doi: 10.1083/jcb.10.4.467.
7
The isolation and preliminary characterization of a major soluble protein of the sea urchin egg.海胆卵一种主要可溶性蛋白的分离与初步鉴定
Exp Cell Res. 1959 Jan;16(1):59-69. doi: 10.1016/0014-4827(59)90195-8.
8
The molecular unit of the microtubules of the mitotic apparatus.有丝分裂器微管的分子单位。
J Mol Biol. 1966 Sep;20(1):75-9. doi: 10.1016/0022-2836(66)90118-5.
9
Studies on sulfhydryl groups during cell division of sea-urchin eggs. 8. Some properties of mitotic apparatus proteins.海胆卵细胞分裂过程中巯基的研究。8. 有丝分裂器蛋白的一些特性。
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