Bryan J
J Cell Biol. 1970 Jun;45(3):606-14. doi: 10.1083/jcb.45.3.606.
The characteristics of the reconstitution of a crystalline component of the sea urchin fertilization membrane are presented. The reassembly of large aggregates of cylindrical or tubular components is effected by the addition of calcium or other divalent cations. The reassembly requires a slightly alkaline pH and is little affected by increasing ionic strength. Reassembly is strongly inhibited by treatment with reducing agents such as dithiothreitol. The role of this protein in the formation of the fertilization membrane and its possible relation to the calcium-insoluble proteins of the mitotic apparatus are discussed.
本文介绍了海胆受精膜晶体成分重构的特征。通过添加钙或其他二价阳离子,可实现圆柱形或管状成分的大聚集体的重新组装。重新组装需要略呈碱性的pH值,且受离子强度增加的影响较小。用二硫苏糖醇等还原剂处理会强烈抑制重新组装。本文还讨论了这种蛋白质在受精膜形成中的作用及其与有丝分裂器中钙不溶性蛋白质的可能关系。