海胆受精膜主要结构成分的分离。
The isolation of a major structural element of the sea urchin fertilization membrane.
作者信息
Bryan J
出版信息
J Cell Biol. 1970 Mar;44(3):635-45. doi: 10.1083/jcb.44.3.635.
Abstract
Procedures for isolating the contents of the cortical granules from the ova of the sea urchin, Strongylocentrotus purpuratus, are reported. Dithiothreitol is used to remove the vitelline coat; the "demembranated" eggs are then subsequently activated with butyric acid. By means of these procedures, the hyaline protein and crystalline or paracrystalline material have been isolated from the cortical granules. The crystalline material consists of sheets of cylinders or tubules 150-200 A in diameter. This material is believed to be a major structural element of the fertilization membrane which, in the absence of the vitelline coat, does not form.
摘要
本文报道了从紫海胆（Strongylocentrotus purpuratus）的卵子中分离皮质颗粒内容物的方法。使用二硫苏糖醇去除卵黄膜；然后用丁酸激活“去膜”卵子。通过这些方法，已从皮质颗粒中分离出透明蛋白和结晶或准结晶物质。结晶物质由直径为150 - 200埃的圆柱或小管片组成。这种物质被认为是受精膜的主要结构成分，在没有卵黄膜的情况下，受精膜不会形成。
相似文献
1
The isolation of a major structural element of the sea urchin fertilization membrane.海胆受精膜主要结构成分的分离。
J Cell Biol. 1970 Mar;44(3):635-45. doi: 10.1083/jcb.44.3.635.
2
The vitelline membrane and cortical particles in sea urchin eggs and their function in maturation and fertilization.海胆卵中的卵黄膜和皮质颗粒及其在成熟和受精过程中的功能。
Adv Morphog. 1966;5:221-325. doi: 10.1016/b978-1-4831-9952-8.50010-9.
3
Sea urchin fertilization envelope: isolation, extraction, and characterization of a major protein fraction from Stronglyocentrotus purpuratus embryos.海胆受精膜：从紫球海胆胚胎中分离、提取和鉴定一种主要蛋白质组分
Biochemistry. 1978 Jun 27;17(13):2605-12. doi: 10.1021/bi00606a023.
4
Fertilization-associated light-scattering changes in eggs of the sea urchin Strongylocentrotus purpuratus.紫海胆卵子中与受精相关的光散射变化
Exp Cell Res. 1971 Apr;65(2):281-8. doi: 10.1016/0014-4827(71)90003-6.
5
The isolation and preliminary analysis of the hyaline layer of sea urchin eggs.海胆卵透明层的分离与初步分析。
Exp Cell Res. 1969 Jan;54(1):140-2. doi: 10.1016/0014-4827(69)90307-3.
6
Intermolecular cross-linking of vitelline envelope polypeptides predominates in the hardened sea urchin fertilization envelope.在硬化的海胆受精膜中，卵黄膜多肽的分子间交联占主导地位。
Gamete Res. 1987 May;17(1):63-75. doi: 10.1002/mrd.1120170108.
7
Sea urchin fertilization envelope: uncoupling of cortical granule exocytosis from envelope assembly and isolation of an envelope intermediate from Strongylocentrotus purpuratus embryos.海胆受精膜：皮层颗粒胞吐作用与膜组装的解偶联以及从紫球海胆胚胎中分离出膜中间体
Dev Biol. 1982 Nov;94(1):252-8. doi: 10.1016/0012-1606(82)90089-6.
8
Direct isolation of the hyaline layer protein released from the cortical granules of the sea urchin egg at fertilization.受精时从海胆卵皮质颗粒释放的透明层蛋白的直接分离。
J Cell Biol. 1970 Jun;45(3):615-22. doi: 10.1083/jcb.45.3.615.
9
Cortical fibers in fertilized eggs of the sea urchin Strongylocentrotus purpuratus.紫海胆受精卵中的皮质纤维。
Exp Cell Res. 1968 Oct;52(2):677-81. doi: 10.1016/0014-4827(68)90509-0.
10
Changes in the topography of the sea urchin egg after fertilization.受精后海胆卵表面形态的变化。
J Cell Biol. 1976 Oct;71(1):35-48. doi: 10.1083/jcb.71.1.35.
引用本文的文献
1
The vitelline layer of the sea urchin egg and its modification during fertilization. A freeze-fracture study using quick-freezing and deep-etching.海胆卵的卵黄膜及其在受精过程中的变化。一项使用快速冷冻和深度蚀刻的冷冻断裂研究。
J Cell Biol. 1980 Mar;84(3):618-32. doi: 10.1083/jcb.84.3.618.
2
A cytological study of the relation of the cortical reaction to subsequent events of fertilization in urethane-treated eggs of the sea urchin, Arbacia punctulata.对经氨基甲酸乙酯处理的海胆（刺冠海胆）卵中皮质反应与后续受精事件关系的细胞学研究。
J Cell Biol. 1970 Dec;47(3):646-65. doi: 10.1083/jcb.47.3.646.
3
On the reconstitution of the crystalline components of the sea urchin fertilization membrane.关于海胆受精膜晶体成分的重构。
J Cell Biol. 1970 Jun;45(3):606-14. doi: 10.1083/jcb.45.3.606.
4
Precipitation of proteins by vinblastine and calcium ions.长春碱和钙离子对蛋白质的沉淀作用。
Proc Natl Acad Sci U S A. 1970 Jul;66(3):807-14. doi: 10.1073/pnas.66.3.807.
5
Assembly of the sea urchin fertilization membrane: isolation of proteoliaisin, a calcium-dependent ovoperoxidase binding protein.海胆受精膜的组装：蛋白酶抑制素的分离，一种钙依赖性卵过氧化物酶结合蛋白。
J Cell Biol. 1985 Mar;100(3):938-46. doi: 10.1083/jcb.100.3.938.
6
Extracellular coats on the surface of Strongylocentrotus purpuratus eggs: stereo electron microscopy of quick-frozen and deep-etched specimens.紫海胆卵表面的细胞外被：速冻和深度蚀刻标本的立体电子显微镜观察
Cell Tissue Res. 1986;246(1):153-61. doi: 10.1007/BF00219012.
7
Regulation of extracellular matrix assembly: in vitro reconstitution of a partial fertilization envelope from isolated components.细胞外基质组装的调控：利用分离成分进行体外部分受精膜的重构。
J Cell Biol. 1987 Jul;105(1):561-7. doi: 10.1083/jcb.105.1.561.
8
Enzyme activities responsible for the defense mechanism of the fertilization envelope.负责受精膜防御机制的酶活性。
Naturwissenschaften. 1991 Apr;78(4):172-3. doi: 10.1007/BF01136206.
9
Enzymatic basis for protection of fish embryos by the fertilization envelope.受精膜对鱼类胚胎保护作用的酶学基础。
Experientia. 1992 Mar 15;48(3):277-81. doi: 10.1007/BF01930475.
10
Changes in the topography of the sea urchin egg after fertilization.受精后海胆卵表面形态的变化。
J Cell Biol. 1976 Oct;71(1):35-48. doi: 10.1083/jcb.71.1.35.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
RELEASE OF ACID POLYSACCHARIDES FOLLOWING FERTILIZATION OF SEA URCHIN EGGS. A CHEMICAL STUDY OF CHANGES IN THE CELL SURFACE.海胆卵受精后酸性多糖的释放。细胞表面变化的化学研究。
Exp Cell Res. 1964 Nov;36:354-67. doi: 10.1016/0014-4827(64)90215-0.
3
Changes in the cortical layer of sea urchin eggs at fertilization as studied with the electron microscope. I. Clypeaster japonicus.用电镜研究海胆卵受精时皮层的变化。I. 日本笠海胆
Exp Cell Res. 1961 Nov;25:383-97. doi: 10.1016/0014-4827(61)90288-9.
4
An electron microscope study of fertilisation of the sea urchin egg Psammechinus miliaris.对海胆卵（粒刺海胆）受精过程的电子显微镜研究。
Exp Cell Res. 1961 Jan;22:45-55. doi: 10.1016/0014-4827(61)90085-4.
5
Acid and base production at fertilization in the sea urchin.海胆受精过程中的酸碱产生
Exp Cell Res. 1961 Jan;22:233-45. doi: 10.1016/0014-4827(61)90101-x.
6
Studies on sulfhydryl groups during cell division of sea urchin egg. II. Mass isolation of the egg cortex and change in its--SH groups during cell division.海胆卵细胞分裂过程中巯基的研究。II. 卵皮质的大规模分离及其在细胞分裂过程中巯基的变化。
J Biophys Biochem Cytol. 1960 Dec;8(3):603-7. doi: 10.1083/jcb.8.3.603.
7
Comparative study of the localization of incorporated 14C-labeled amino acids and 35SO4 in the sea urchin ovary, egg and embryo.海胆卵巢、卵和胚胎中掺入的14C标记氨基酸和35SO4定位的比较研究。
Exp Cell Res. 1961 Aug;24:356-78. doi: 10.1016/0014-4827(61)90438-4.
8
The isolation and preliminary characterization of a major soluble protein of the sea urchin egg.海胆卵一种主要可溶性蛋白的分离与初步鉴定
Exp Cell Res. 1959 Jan;16(1):59-69. doi: 10.1016/0014-4827(59)90195-8.
9
The fine structure of cortical granules in eggs and gastrulae of Mytilus edulis.紫贻贝卵子和原肠胚中皮质颗粒的精细结构。
J Ultrastruct Res. 1967 Feb;17(3):314-26. doi: 10.1016/s0022-5320(67)80051-0.
10
Beta-1,3-glucanase of sea urchin eggs: release from particles at fertilization.海胆卵的β-1,3-葡聚糖酶：受精时从颗粒中释放出来。
Science. 1969 Jan 17;163(3864):294-6. doi: 10.1126/science.163.3864.294.
Zotero 插件