药物的高压液相色谱法。II. 微粒阳离子交换柱的评估

High-pressure liquid chromatography of drugs. II. An evaluation of a microparticulate cation-exchange column.

作者信息

Twitchett P J, Gorvin A E, Moffat A C

出版信息

J Chromatogr. 1976 May 26;120(2):359-68. doi: 10.1016/s0021-9673(76)80013-1.

Abstract

A microparticulate cation-exchange column has been evaluated for the chromatography of thirty compounds selected as representative of a wide variety of drug substances. Although the column exhibited a strong partition effect besides the expected ion-exchange mechanism, the retention of drugs could be predictably influenced by variation of the eluent ionic strength and organic solvent content. For acidic drugs the column showed little selectivity (although a salting-out effect increased retention at high eluent ionic strengths), but for basic substances Partisil SCX may afford a useful separative medium offering reasonable chromatographic efficiency (HETP is approximately 0.1 mm). The column longevity, however, is at present questionable.

摘要

已对一种微粒阳离子交换柱进行了评估，用于对30种被选作多种药物代表的化合物进行色谱分析。尽管该柱除了预期的离子交换机制外还表现出很强的分配效应，但药物的保留情况可通过改变洗脱液离子强度和有机溶剂含量来进行预测。对于酸性药物，该柱选择性很小（尽管在高洗脱液离子强度下盐析效应会增加保留时间），但对于碱性物质，Partisil SCX柱可能提供一种有用的分离介质，具有合理的色谱效率（理论塔板高度约为0.1mm）。然而，目前该柱的使用寿命存在疑问。