药物的高压液相色谱法。十八烷基硅烷固定相的评估。

High-pressure liquid chromatography of drugs. An evaluation of an octadecylsilane stationary phase.

作者信息

Twitchett P J, Moffat A C

出版信息

J Chromatogr. 1975 Aug 20;111(1):149-57. doi: 10.1016/s0021-9673(01)80156-4.

Abstract

The performance of a commonly used high-pressure liquid chromatographic stationary phase, octadecylsilane, has been evaluated for 30 compounds selected as representative of a wide variety of drug substances. Chromatographic behavior is found to be highly predictable on the basis of pKa and partition coefficient, and the stationary phase should be especially valuable for the separation of acidic and neutral drugs. For basic drugs, however, the column efficiency is poor, detracting from the overall usefulness.

摘要

已对一种常用的高压液相色谱固定相十八烷基硅烷针对30种被选作各种药物代表的化合物进行了评估。基于pKa和分配系数，发现色谱行为具有高度可预测性，并且该固定相对酸性和中性药物的分离应特别有价值。然而，对于碱性药物，柱效较差，降低了其整体实用性。