与无机载体共价偶联的抗原和抗体吸附剂的制备与表征
Preparation and characterization of antigen and antibody adsorbents covalently coupled to an inorganic carrier.
作者信息
Weetall H H
出版信息
Biochem J. 1970 Apr;117(2):257-61. doi: 10.1042/bj1170257.
Abstract
Immunoadsorbents were prepared by the use of an inorganic carrier. The proteins were covalently coupled to the carrier through a silane coupling agent. An immunoadsorbent prepared with human gamma-globulin was found to complex with specific antibodies. These antibodies could be recovered in high yield and purity from the adsorbent. Similarly, insolubilized antibodies to l-asparaginase were used to isolate and purify the enzyme from crude extracts. These insolubilized antibodies are extremely stable with time and may be reused many times.
摘要
使用无机载体制备免疫吸附剂。蛋白质通过硅烷偶联剂与载体共价偶联。发现用人γ-球蛋白制备的免疫吸附剂能与特异性抗体形成复合物。这些抗体可以从吸附剂中高产量、高纯度地回收。同样，用对L-天冬酰胺酶的固定化抗体从粗提物中分离和纯化该酶。这些固定化抗体随时间极其稳定，可多次重复使用。
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