Zaĭtseva G N, Iakunin A F, Maslov D A, Kuz'min E V, Zograf Iu N
Biokhimiia. 1981 Nov;46(11):2056-63.
The ability of a complex associate made up of maxi- and mini-circular molecules of kinetoplast DNA from C oncopelti to perform polypeptide synthesis in a cell-free system of conjugate transcription and translation of E. coli was demonstrated. The coding function of maxi-circular molecules and genetic inertness of mini-circular molecules of kinetoplast DNA during polypeptide synthesis in vitro was found. Total RNA from the kinetoplasts was shown to undergo translation in cell-free protein-synthesizing system of E. coli to form 9-10 polypeptides. The molecular weights of many polypeptides, whose synthesis in this system is performed by DNA and RNA from Kinetoplasts were found to be similar.
已证明，由来自锥蝽动质体DNA的大、小环状分子组成的复合缔合体，能够在大肠杆菌的无细胞转录翻译偶联系统中进行多肽合成。发现在体外多肽合成过程中，动质体DNA的大环状分子具有编码功能，而小环状分子具有遗传惰性。已表明，来自动质体的总RNA在大肠杆菌的无细胞蛋白质合成系统中能够进行翻译，从而形成9 - 10种多肽。还发现，在该系统中由动质体的DNA和RNA合成的许多多肽，其分子量相似。
