培养的中国仓鼠细胞密度随有丝分裂周期阶段的不变性。
Density invariance of cultured Chinese hamster cells with stage of the mitotic cycle.
作者信息
Anderson E C, Petersen D F, Tobey R A
出版信息
Biophys J. 1970 Jul;10(7):630-45. doi: 10.1016/S0006-3495(70)86325-1.
Abstract
Isopycnic banding of Chinese hamster line CHO cells in Ficoll gradients shows that a population in balanced, exponential growth is very homogeneous with respect to density, the coefficient of variation of the density distribution spectrum being less than 5% of the mean reduced density (i.e. density minus one). Similar measurements on synchronized cultures indicate that reduced density varies by less than 2% around the life cycle. The mean density of CHO cells in F-10 growth medium is calculated to be 1.051 after correction for osmotic effects of the Ficoll gradient.
摘要
中国仓鼠CHO细胞系在菲可密度梯度离心中的等密度沉降显示，处于平衡指数生长状态的细胞群体在密度方面非常均匀，密度分布谱的变异系数小于平均折光密度（即密度减去1）的5%。对同步培养物进行的类似测量表明，在整个生命周期中，折光密度的变化小于2%。经菲可密度梯度渗透压效应校正后，计算得出F-10生长培养基中CHO细胞的平均密度为1.051。
相似文献
1
Density invariance of cultured Chinese hamster cells with stage of the mitotic cycle.培养的中国仓鼠细胞密度随有丝分裂周期阶段的不变性。
Biophys J. 1970 Jul;10(7):630-45. doi: 10.1016/S0006-3495(70)86325-1.
2
Animal cells: noncorrelation of length of G1 phase with size after mitosis.
Science. 1970 Jan 2;167(3914):80-2. doi: 10.1126/science.167.3914.80.
3
The effect of potassium on the cell membrane potential and the passage of synchronized cells through the cell cycle.
J Cell Physiol. 1975 Apr;85(2 Pt 1):283-91. doi: 10.1002/jcp.1040850215.
4
Instantaneous evaluation of mammalian cell culture growth rates through analysis of the mitotic index.通过分析有丝分裂指数对哺乳动物细胞培养生长速率进行即时评估。
J Biotechnol. 2002 Apr 25;95(1):63-84. doi: 10.1016/s0168-1656(01)00444-8.
5
6
Isolated mammalian metaphase chromosomes. II. Fractionated chromosomes of mouse and Chinese hamster cells.分离的哺乳动物中期染色体。II. 小鼠和中国仓鼠细胞的分级染色体。
J Mol Biol. 1969 Mar 14;40(2):203-16. doi: 10.1016/0022-2836(69)90469-0.
7
8
9
[Duration of the mitotic cycle of Chinese hamster cells cultured at 30--39 degrees].[在30 - 39摄氏度培养的中国仓鼠细胞有丝分裂周期的持续时间]
Biull Eksp Biol Med. 1976;82(11):1365-7.
10
Separation of cells exhibiting acid phosphatase activity from disaggregated hamster prostate cells in an isokinetic gradient of Ficoll in tissue culture medium.在组织培养基中菲可的等动力学梯度下，从分散的仓鼠前列腺细胞中分离出表现出酸性磷酸酶活性的细胞。
J Natl Cancer Inst. 1975 Jan;54(1):147-50. doi: 10.1093/jnci/54.1.147.
引用本文的文献
1
Exploring operational boundaries for acoustic concentration of cell suspensions.探索细胞悬浮液声聚集的操作边界。
Appl Microbiol Biotechnol. 2024 Jun 19;108(1):387. doi: 10.1007/s00253-024-13215-1.
2
A Four-Step Purification Process for Gag VLPs: From Culture Supernatant to High-Purity Lyophilized Particles.用于Gag病毒样颗粒的四步纯化工艺：从培养上清液到高纯度冻干颗粒
Vaccines (Basel). 2021 Oct 9;9(10):1154. doi: 10.3390/vaccines9101154.
3
The Relative Densities of Cytoplasm and Nuclear Compartments Are Robust against Strong Perturbation.细胞质和核区室的相对密度对强烈扰动具有稳健性。
Biophys J. 2020 Nov 17;119(10):1946-1957. doi: 10.1016/j.bpj.2020.08.044. Epub 2020 Oct 20.
4
On-chip surface acoustic wave and micropipette aspiration techniques to assess cell elastic properties.用于评估细胞弹性特性的片上表面声波和微量移液器抽吸技术。
Biomicrofluidics. 2020 Feb 18;14(1):014114. doi: 10.1063/1.5138662. eCollection 2020 Jan.
5
On-chip cell mechanophenotyping using phase modulated surface acoustic wave.利用相位调制表面声波进行芯片上的细胞机械表型分析。
Biomicrofluidics. 2019 Apr 23;13(2):024107. doi: 10.1063/1.5084297. eCollection 2019 Mar.
6
Dielectrophoresis study of temporal change in internal conductivity of single CHO cells after electroporation by pulsed electric fields.脉冲电场电穿孔后单个CHO细胞内部电导率随时间变化的介电泳研究
Biomicrofluidics. 2017 Feb 13;11(1):014111. doi: 10.1063/1.4975978. eCollection 2017 Jan.
7
Dielectric model for Chinese hamster ovary cells obtained by dielectrophoresis cytometry.通过介电泳细胞术获得的中国仓鼠卵巢细胞的介电模型。
Biomicrofluidics. 2016 Jan 21;10(1):014111. doi: 10.1063/1.4940432. eCollection 2016 Jan.
8
Combining AFM and acoustic probes to reveal changes in the elastic stiffness tensor of living cells.结合原子力显微镜和声学探头揭示活细胞弹性刚度张量的变化。
Biophys J. 2014 Oct 7;107(7):1502-12. doi: 10.1016/j.bpj.2014.07.073.
9
Oxygen transport and stem cell aggregation in stirred-suspension bioreactor cultures.搅拌悬浮式生物反应器培养中的氧气传输与干细胞聚集
PLoS One. 2014 Jul 17;9(7):e102486. doi: 10.1371/journal.pone.0102486. eCollection 2014.
10
Contribution of stochastic partitioning at human embryonic stem cell division to NANOG heterogeneity.人类胚胎干细胞分裂时的随机分区对 NANOG 异质性的贡献。
PLoS One. 2012;7(11):e50715. doi: 10.1371/journal.pone.0050715. Epub 2012 Nov 30.
本文引用的文献
1
A THREE-COMPONENT THEORY OF SEDIMENTATION EQUILIBRIUM IN A DENSITY GRADIENT.密度梯度中沉降平衡的三组分理论
Proc Natl Acad Sci U S A. 1961 Jul;47(7):999-1004. doi: 10.1073/pnas.47.7.999.
2
[Interference microscopic studies on the growth of individual cells (HeLa cells) in tissue culture].[组织培养中单个细胞（海拉细胞）生长的干涉显微镜研究]
Frankf Z Pathol. 1960;70:271-99.
3
THE DISTRIBUTION OF BUOYANT DENSITY OF HUMAN ERYTHROCYTES IN BOVINE ALBUMIN SOLUTIONS.人红细胞在牛白蛋白溶液中的浮力密度分布
Proc Natl Acad Sci U S A. 1964 Mar;51(3):520-8. doi: 10.1073/pnas.51.3.520.
4
MITOTICALLY SYNCHRONIZED MAMMALIAN CELLS: A SIMPLE METHOD FOR OBTAINING LARGE POPULATIONS.有丝分裂同步的哺乳动物细胞：一种获取大量细胞群体的简单方法。
Science. 1964 May 29;144(3622):1152-3. doi: 10.1126/science.144.3622.1152.
5
Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells.同步分裂的海拉细胞群体中的生长与核酸合成
Exp Cell Res. 1963 Apr;30:344-62. doi: 10.1016/0014-4827(63)90306-9.
6
Growth, cell and nuclear divisions in some bacteria.某些细菌中的生长、细胞分裂和核分裂
J Gen Microbiol. 1962 Nov;29:421-34. doi: 10.1099/00221287-29-3-421.
7
Rate of growth of Bacillus cereus between divisions.蜡样芽孢杆菌分裂间期的生长速率。
J Gen Microbiol. 1962 Apr;28:15-33. doi: 10.1099/00221287-28-1-15.
8
Osmotic properties of living cells.活细胞的渗透特性。
Int Rev Cytol. 1959;8:387-448. doi: 10.1016/s0074-7696(08)62736-9.
9
The growth of single cells. III. Streptococcus faecalis.单细胞的生长。III. 粪链球菌
Exp Cell Res. 1961 Jan;22:208-25. doi: 10.1016/0014-4827(61)90099-4.
10
Dependency on medium and temperature of cell size and chemical composition during balanced grown of Salmonella typhimurium.鼠伤寒沙门氏菌平衡生长期间细胞大小和化学成分对培养基及温度的依赖性。
J Gen Microbiol. 1958 Dec;19(3):592-606. doi: 10.1099/00221287-19-3-592.
Zotero 插件