通过介电泳细胞术获得的中国仓鼠卵巢细胞的介电模型。
Dielectric model for Chinese hamster ovary cells obtained by dielectrophoresis cytometry.
作者信息
Salimi E, Braasch K, Butler M, Thomson D J, Bridges G E
机构信息
Department of Electrical and Computer Engineering, University of Manitoba , Winnipeg, Manitoba R3T 5V6, Canada.
Department of Microbiology, University of Manitoba , Winnipeg, Manitoba R3T 2N2, Canada.
出版信息
Biomicrofluidics. 2016 Jan 21;10(1):014111. doi: 10.1063/1.4940432. eCollection 2016 Jan.
Abstract
We present a dielectric model and its parameters for Chinese hamster ovary (CHO) cells based on a double-shell structure which includes the cell membrane, cytoplasm, nuclear envelope, and nucleoplasm. Employing a dielectrophoresis (DEP) based technique and a microfluidic system, the DEP response of many single CHO cells is measured and the spectrum of the Clausius-Mossotti factor is obtained. The dielectric parameters of the model are then extracted by curve-fitting to the measured spectral data. Using this approach over the 0.6-10 MHz frequency range, we report the values for CHO cells' membrane permittivity, membrane thickness, cytoplasm conductivity, nuclear envelope permittivity, and nucleoplasm conductivity. The size of the cell and its nuclei are obtained using optical techniques.
摘要
我们基于包含细胞膜、细胞质、核膜和核质的双壳结构，提出了一种针对中国仓鼠卵巢（CHO）细胞的介电模型及其参数。采用基于介电泳（DEP）的技术和微流控系统，测量了许多单个CHO细胞的DEP响应，并获得了克劳修斯-莫索蒂因子的频谱。然后通过对测量的光谱数据进行曲线拟合来提取模型的介电参数。在0.6 - 10 MHz频率范围内使用这种方法，我们报告了CHO细胞膜电容率、膜厚度、细胞质电导率、核膜电容率和核质电导率的值。使用光学技术获得细胞及其细胞核的大小。
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