Mazzini M N, Cerezo A S, De Cerezo J M
J Chromatogr. 1979 May 21;173(2):365-71. doi: 10.1016/s0021-9673(00)92305-7.
Elution diagrams obtained on affinity chromatography show that the antigenic fractions of human seminal plasma studied, namely (a) the non-dialyzable components of human seminal plasma and (b) its trichloroacetic acid-soluble fraction, (c) the trichloroacetic acid-soluble fraction of whole human seminal plasma and (d) the pronase digested human seminal plasma, are complex mixtures of glycoproteins with minor amounts of polysaccharides. Some of these glycoproteins contain significant percentages of carbohydrates while others contain only trace amounts. Most of the glycoproteins carry non-reducing end-chain groups comprising alpha-D-glucopyranosyl, alpha-D-mannopyranosyl or sterically related residues.
亲和层析得到的洗脱图表明，所研究的人类精浆抗原部分，即（a）人类精浆的不可透析成分，（b）其可溶于三氯乙酸的部分，（c）整个人类精浆的可溶于三氯乙酸的部分，以及（d）经链霉蛋白酶消化的人类精浆，是糖蛋白与少量多糖的复杂混合物。其中一些糖蛋白含有相当比例的碳水化合物，而另一些仅含微量。大多数糖蛋白带有由α-D-吡喃葡萄糖基、α-D-吡喃甘露糖基或空间相关残基组成的非还原末端链基团。
