de Cerezo J M, Bueno M P, Skowroñski B, Cerezo A S
J Reprod Immunol. 1982 Feb;4(1):11-21. doi: 10.1016/0165-0378(82)90019-5.
The seminal plasma and a fraction of the spermatozoal membrane solubilised by sarkosyl contain similar antigenic determinants. The glycoprotein isolated from the seminal plasma also contains determinants which are immunologically identical to those of the sarkosyl soluble fraction. Three of these determinants were identified as: two oligosaccharide chains with terminal N-acetyl neuraminic acid and with subterminal alpha-L-fucopyranose and beta-D-galactopyranose residues respectively, and an oligosaccharide chain with beta-D-galactopyranose as non-reducing end-chain. The receptors on the spermatozoa to anti-glycoprotein and anti-seminal plasma antibodies are localized. The specificity of the receptors localised in the neck region depends entirely on the presence of N-acetyl neuraminic acid as end-chain group while the specificity of the receptors to the anti-seminal plasma antibodies in the intermediate segment plus tail and to the anti-glycoprotein antibodies in the post-nuclear cap depends also on the subterminal units. At least two other receptors in the equatorial zone are ended by residues different from the N-acetyl neuraminic acid. One of them, which is also found in the acrosome, does not have specificity for anti-glycoprotein antibodies, while the other, which is not in the acrosome, has anti-glycoprotein antibodies specificity and may have a terminal beta-D-galactopyranose residue.
精浆以及被十二烷基肌氨酸钠溶解的部分精子细胞膜含有相似的抗原决定簇。从精浆中分离出的糖蛋白也含有与十二烷基肌氨酸钠可溶部分在免疫学上相同的决定簇。其中三个决定簇被鉴定为：两条分别带有末端N-乙酰神经氨酸以及亚末端α-L-呋喃岩藻糖和β-D-吡喃半乳糖残基的寡糖链，以及一条以β-D-吡喃半乳糖作为非还原端链的寡糖链。精子上抗糖蛋白和抗精浆抗体的受体得以定位。位于颈部区域的受体的特异性完全取决于作为端链基团的N-乙酰神经氨酸的存在，而位于中段加尾部的抗精浆抗体受体以及位于核后帽的抗糖蛋白抗体受体的特异性还取决于亚末端单元。赤道区至少还有另外两种受体，其末端残基不同于N-乙酰神经氨酸。其中一种在顶体中也有发现，对抗糖蛋白抗体不具有特异性，而另一种不在顶体中，具有抗糖蛋白抗体特异性，可能带有末端β-D-吡喃半乳糖残基。
