使用双缩脲反应和铜-葡聚糖凝胶反应器连续检测肽和蛋白质的灵敏方法（作者译）

[Sensitive method for continuous detection of peptides and proteins using the biuret reaction and a copper-Sephadex reactor (author's transl)].

作者信息

Later R, Quincy C

出版信息

J Chromatogr. 1979 Jun 1;174(1):131-41. doi: 10.1016/s0021-9673(00)87043-0.

Abstract

We describe a detection method relying both on the copper displacement from a Sephadex gel by peptides and proteins, and on the subsequent colorimetric determination of the complexed copper. The system described is fully automated and it permits a continuous analysis of column effluents. The choice of cuprizone as a detecting reagent for copper, enables one to bring the detection limit down to 200 ng for albumin and 60 ng for alanylglycylglycin. The specificity of the method is the same as the biuret reaction. Some examples of the possible applications are given.

摘要

我们描述了一种检测方法，该方法既依赖于肽和蛋白质从葡聚糖凝胶中置换铜，又依赖于随后对络合铜的比色测定。所描述的系统是完全自动化的，它允许对柱流出物进行连续分析。选择铜试剂作为铜的检测试剂，可将白蛋白的检测限降至200 ng，丙氨酰甘氨酰甘氨酸的检测限降至60 ng。该方法的特异性与双缩脲反应相同。文中给出了一些可能应用的示例。