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[Usage of the gel filtration method for the determination of urinary proteins (author's transl)].

作者信息

Marsili G, Tacconi R, Spurio G, Cipriani S

出版信息

Quad Sclavo Diagn. 1979 Sep;15(3):809-15.

PMID:233432
Abstract

The urinary proteins determination was carried out after purification by gel filtration over Sephadex rosin. The proteins eluaded react with Biuret-reagent to establish the coloured copper-proteins complex. If proteins concentrations is inferior, the proteins can be determinated by the copper-proteins complex isolation with second gel filtration and titrating Cu++ with reactives: dietildithiocarbamate or the PAR (4-(2pyridyl-azo)resorcinol monosodium salt).

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