生物聚合物的高速空间排阻色谱法。

High-speed steric exclusion chromatography of biopolymers.

作者信息

Wehr C T, Abbott S R

出版信息

J Chromatogr. 1979 Dec 20;185:453-462. doi: 10.1016/s0021-9673(00)85621-6.

Abstract

Biopolymer separations were studied on Micropak TSK type SW columns, which contain an aqueous compatible steric exclusion support. Columns of two different pore sizes, designated 2000SW and 3000SW, were compared for separation of proteins and nucleic acids covering a molecular weight range of 13,500 to 340,000 and the effect of molecular shape and denaturation upon elution volume was investigated. Use of high-speed steric exclusion chromatography as a prefractionation step prior to ion-exchange chromatography in biopolymer purification schemes is discussed.

摘要

在含有与水相容的空间排阻载体的Micropak TSK型SW柱上研究了生物聚合物的分离。比较了两种不同孔径的柱，分别命名为2000SW和3000SW，用于分离分子量范围为13,500至340,000的蛋白质和核酸，并研究了分子形状和变性对洗脱体积的影响。讨论了在生物聚合物纯化方案中，使用高速空间排阻色谱作为离子交换色谱之前的预分级步骤。