Regnier F E
Science. 1983 Oct 21;222(4621):245-52. doi: 10.1126/science.6353575.
The ability to separate biological macromolecules with good resolution on liquid chromatographic columns has depended on the development of suitable packing materials. In size exclusion chromatography, molecules are separated by size on the basis of differential permeation of the packing. Ion exchange, hydrophobic interaction (or reversed-phase), and affinity chromatography are all surface-mediated separation methods, although they depend on different retention mechanisms. High-performance liquid chromatographic columns designed for biopolymers offer major advantages over conventional columns in both speed and resolving power. The exponential growth of literature on the high-performance separation of peptides and proteins in particular indicates that the technique will become the dominant form of column liquid chromatography.
在液相色谱柱上以良好分辨率分离生物大分子的能力取决于合适填充材料的开发。在尺寸排阻色谱中，分子基于填充材料的差异渗透按大小分离。离子交换、疏水相互作用（或反相）和亲和色谱都是表面介导的分离方法，尽管它们依赖于不同的保留机制。为生物聚合物设计的高效液相色谱柱在速度和分离能力方面都比传统色谱柱具有主要优势。特别是关于肽和蛋白质高效分离的文献呈指数增长，这表明该技术将成为柱液相色谱的主导形式。
