Eriksson B M, Persson B A, Lindberg M
J Chromatogr. 1979 Dec 20;185:575-81. doi: 10.1016/s0021-9673(00)85631-9.
Apomorphine is extracted from plasma or tissue homogenate with ethyl acetate. After back-extraction into hydrochloric acid, the apomorphine is extracted as an ion pair with 3,5-di-tert.-butyl-2-hydroxybenzene sulphonate into a small volume of methylene chloride and the solution is injected into the chromatographic column. Apomorphine is separated on microporous silica with a mixture of aqueous perchloric acid, methanol and methylene chloride as the mobile phase. With absorbance measurement of the eluent at 254 nm the method permits the determination of 15 pmol of apomorphine in 1 ml of plasma or in a rat brain. The coefficient of variation was 4% at the 100 pmol level.
阿扑吗啡用乙酸乙酯从血浆或组织匀浆中提取。反萃取到盐酸中后，阿扑吗啡与3,5-二叔丁基-2-羟基苯磺酸盐形成离子对，萃取到少量二氯甲烷中，然后将溶液注入色谱柱。阿扑吗啡在微孔硅胶上分离，流动相为高氯酸水溶液、甲醇和二氯甲烷的混合物。通过在254nm处测量洗脱液的吸光度，该方法可测定1ml血浆或大鼠脑中15pmol的阿扑吗啡。在100pmol水平时变异系数为4%。
