Baaske D M, Keiser J E, Smith R V
J Chromatogr. 1977 Oct 1;140(1):57-64. doi: 10.1016/s0021-9673(00)83601-8.
A method is described for the analysis of 1 to 10 microgram/ml concentrations of apomorphine in plasma. The procedure is based on ethyl acetate extraction, a back extraction cleanup-step, derivatization with heptafluorobutyric anhydride, and gas chromatography on a 3% OV-17 column using flame ionization detection. N-n-Propylnorapomorphine is employed as an internal standard and quantitative relative recoveries of drug are realized with relative standard deviations of 4.6%. The method permits analysis of apomorphine in the presence of its two monomethyl ether metabolites, apocodeine and isoapocodeine. The latter compounds are also chromatographically resolved as their heptofluorobutyrate derivatives.
本文描述了一种分析血浆中浓度为1至10微克/毫升的阿扑吗啡的方法。该方法基于乙酸乙酯萃取、反萃取净化步骤、用七氟丁酸酐衍生化，以及在3% OV - 17柱上使用火焰离子化检测的气相色谱法。N - 正丙基去甲阿扑吗啡用作内标，药物的定量相对回收率实现，相对标准偏差为4.6%。该方法允许在其两种单甲醚代谢物阿朴可待因和异阿朴可待因存在的情况下分析阿扑吗啡。后两种化合物也作为其七氟丁酸酯衍生物通过色谱法分离。
