Stein W, Bohner J, Eggstein M, Lang H
J Clin Chem Clin Biochem. 1979 Nov;17(11):739-45.
Sera from seven-patients with an unusual high ratio of creatine kinase-MB versus total creatine kinase, as found in the immunoinhibition test, were investigated. In every case, the electrophoretic separation of the isoenzymes showed a slow moving band, and exclusion chromatography showed the presence of macro-creatine kinase. Further chromatographic, electrophoretic and immunological investigations indicated that the macro-creatine kinases in six cases are complexes of creatine kinase-BB linked to immunoglobulin G.
对7例患者的血清进行了研究，这些患者在免疫抑制试验中肌酸激酶-MB与总肌酸激酶的比例异常高。在每种情况下，同工酶的电泳分离均显示出一条迁移缓慢的条带，排阻色谱法显示存在巨肌酸激酶。进一步的色谱、电泳和免疫学研究表明，6例患者的巨肌酸激酶是与免疫球蛋白G相连的肌酸激酶-BB复合物。
