Bickerstaffe R, Annison E F
Biochem J. 1970 Jul;118(3):433-42. doi: 10.1042/bj1180433.
Comparative studies were made of the uptake and metabolism of cis- and trans-octadecenoic acids by the perfused chicken liver. No differences were observed in the rates of uptake of the isomers. There was considerable incorporation of radioactivity into triglycerides and phospholipids, and some release of labelled lipid into the perfusate was observed. The cis-fatty acids were more readily incorporated into triglycerides than phospholipids, the reverse being true of the trans-fatty acids. Examination of the intramolecular distribution of fatty acids in triglycerides showed that the trans-fatty acid and stearate mainly occupied the 1- and 3-positions, and cis-fatty acids the 2-position. In the phospholipids phosphatidylcholine and phosphatidylethanolamine the trans-fatty acids again behaved like stearic acid and favoured the 1-position. No evidence was obtained of atypical patterns of uptake or metabolism of the trans-fatty acids.
对灌注鸡肝摄取和代谢顺式和反式十八碳烯酸的情况进行了比较研究。未观察到异构体摄取速率的差异。放射性大量掺入甘油三酯和磷脂中,并且观察到有一些标记脂质释放到灌注液中。顺式脂肪酸比磷脂更容易掺入甘油三酯中,反式脂肪酸则相反。对甘油三酯中脂肪酸的分子内分布进行检查表明,反式脂肪酸和硬脂酸主要占据1位和3位,顺式脂肪酸占据2位。在磷脂酰胆碱和磷脂酰乙醇胺中,反式脂肪酸的行为再次类似于硬脂酸,倾向于占据1位。未获得反式脂肪酸摄取或代谢的非典型模式的证据。