Bickerstaffe R, West C E, Annison E F
Biochem J. 1970 Jul;118(3):427-31. doi: 10.1042/bj1180427.
A procedure for the perfusion of the isolated chicken liver was developed. The preparation satisfied many of the criteria of normal physiological function, e.g. oxygen consumption and the absence in the perfusion medium of enzymes indicative of cell damage, and retained its capacity to synthesize lipids from glucose, acetate and long-chain fatty acids. Part of newly synthesized triglyceride was released into the perfusion medium as lipoprotein.
开发了一种用于灌注离体鸡肝的方法。该制剂满足许多正常生理功能的标准,例如耗氧量以及灌注介质中不存在指示细胞损伤的酶,并且保留了从葡萄糖、乙酸盐和长链脂肪酸合成脂质的能力。新合成的甘油三酯的一部分作为脂蛋白释放到灌注介质中。