粗糙脉孢菌摄取钴离子的机制。
The mechanism of uptake of cobalt ions by Neurospora crassa.
作者信息
Venkateswerlu G, Sastry K S
出版信息
Biochem J. 1970 Jul;118(3):497-503. doi: 10.1042/bj1180497.
Uptake of Co(2+) by 3-day-old mycelia of Neurospora crassa involves cell-surface binding as well as transport into the intracellular space. The surface binding is rapid and accounts for 30-40% of the total Co(2+) uptake. Transport of Co(2+) occurs at a rate of 40mug/h per 100mg dry wt. Surface binding and overall uptake show different temperature dependence. Metabolic inhibitors such as azide, dinitrophenol and fluoride depress transport of Co(2+). The overall uptake of Co(2+) exhibits a high K(m) value and hence the concentration mechanism is one of low ;affinity' for the metal. The uptake of Co(2+) varies linearly with pH in the range pH3 to pH6. Mg(2+) inhibits both surface binding and transport of Co(2+). It is suggested that the system that transports Mg(2+) is also involved in Co(2+) uptake by N. crassa.
粗糙脉孢菌3日龄菌丝体对Co(2+)的摄取涉及细胞表面结合以及向细胞内空间的转运。表面结合迅速，占总Co(2+)摄取量的30 - 40%。Co(2+)的转运速率为每100mg干重40μg/h。表面结合和总体摄取表现出不同的温度依赖性。叠氮化物、二硝基苯酚和氟化物等代谢抑制剂会抑制Co(2+)的转运。Co(2+)的总体摄取表现出高K(m)值，因此其浓缩机制对该金属的“亲和力”较低。在pH3至pH6范围内，Co(2+)的摄取随pH呈线性变化。Mg(2+)抑制Co(2+)的表面结合和转运。有人提出，转运Mg(2+)的系统也参与粗糙脉孢菌对Co(2+)的摄取。
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