甾体生物合成抑制剂对兔卵巢卵泡中前列腺素形成的影响。

Effects of inhibitors of steroid biosynthesis on prostaglandin formation in rabbit ovarian follicles.

作者信息

Patwardhan V V, Lanthier A

出版信息

Prostaglandins. 1979 Dec;18(6):859-67. doi: 10.1016/0090-6980(79)90123-0.

Abstract

Rabbit ovarian follicles were incubated without stimulation, with LH and with LH + an inhibitor or steroid biosynthesis. Formation of prostaglandins PGE and PGF and of progesterone and estradiol was measured in these incubates. It was found that aminoglutethimide phosphate (AGP) inhibited the LH stimulated biosynthesis of both prostaglandins and steroids. However U 30870 and Metyrapone, while completely inhibiting the LH stimulated biosynthesis of progesterone and estradiol respectively, had no effect on the formation of prostaglandins. Further, the inhibition of prostaglandin formation by AGP could not be reversed by exogenou steroids. It, therefore, appears that the effect of AGP on prostaglandin biosynthesis may not be related to its effect on steroid biosynthesis. However, the response of rabbit follicles to AGP is contrary to that reported for rat follicles and indicates different control mechanisms for prostaglandin formation in the follicles of the two species.

摘要

将兔卵巢卵泡分别在无刺激、促黄体生成素（LH）刺激以及LH + 一种抑制剂或甾体生物合成抑制剂的条件下进行孵育。测定这些孵育物中前列腺素PGE和PGF以及孕酮和雌二醇的生成情况。结果发现，磷酸氨基乙腈（AGP）抑制LH刺激的前列腺素和甾体的生物合成。然而，U 30870和甲吡酮虽然分别完全抑制LH刺激的孕酮和雌二醇的生物合成，但对前列腺素的生成没有影响。此外，外源性甾体不能逆转AGP对前列腺素生成的抑制作用。因此，AGP对前列腺素生物合成的作用可能与其对甾体生物合成的作用无关。然而，兔卵泡对AGP的反应与大鼠卵泡的报道相反，这表明两种动物卵泡中前列腺素生成的调控机制不同。