Dhanotiya R S, Srivastava R K
Ann Rech Vet. 1979;10(4):557-65.
Experiments have been conducted to estimate the fructolytic index, Fl (mg of fructose utilized by 10(9) spermatozoa in 60 minutes), Fructolytic Coefficient, Ku (Fractional decline in the spermatozoal fructolytic activity on an instantaneous basis), Maximum fructose utilization, MFU (mg of fructose utilized by 10(9) spermatozoa in one minute at zero incubation time on an instantaneous basis) and T 1/2 (Biological half life of semen) of buffalo spermatozoa. The effect of citrate and phosphate buffers on the measures of fructolysis, the Pasteur effect and the fructose utilization by the head, midpiece and tail fraction of spermatozoa have also been investigated. Fructolytic index of the buffalo spermatozoa has been compared with those of exotic bulls. Fl was highly significantly different between first, second and third hour of incubation. Significant correlations were obtained between sperm concentration and fructose utilization in all the 20 minute periods. Ku was independent of initial fructose concentration (IFC) sperm concentration and the incubation period. The Fructose utilization followed a first order reaction. The correlation coefficient between the seminal attributes and different measures of fructolysis have been presented. All the measures of fructolysis were significantly higher in citrate buffer as compared to phosphate buffer. None of the fractions of the spermatozoa showed fructolytic activity on incubation with exogeneous fructose.
已开展实验来估算水牛精子的果糖分解指数（Fl，即10⁹个精子在60分钟内利用的果糖毫克数）、果糖分解系数（Ku，即精子果糖分解活性的瞬间分数下降）、最大果糖利用率（MFU，即10⁹个精子在零孵育时间时瞬间每分钟利用的果糖毫克数）以及精液的生物半衰期（T 1/2）。还研究了柠檬酸盐和磷酸盐缓冲液对果糖分解指标、巴斯德效应以及精子头部、中段和尾部片段果糖利用的影响。已将水牛精子的果糖分解指数与外来公牛的进行了比较。在孵育的第一、第二和第三小时，Fl存在极显著差异。在所有20分钟时间段内，精子浓度与果糖利用之间均存在显著相关性。Ku与初始果糖浓度（IFC）、精子浓度和孵育期无关。果糖利用遵循一级反应。已给出精液属性与不同果糖分解指标之间的相关系数。与磷酸盐缓冲液相比，在柠檬酸盐缓冲液中所有果糖分解指标均显著更高。精子的任何片段与外源性果糖孵育时均未显示出果糖分解活性。
