Sánchez-Marroquín A, Ledezma M, Carreño R
Appl Microbiol. 1970 Nov;20(5):687-92. doi: 10.1128/am.20.5.687-692.1970.
The extracellular production of l-lysine in media with cane sugar, blackstrap molasses, or clarified sugar-cane juice by a previously obtained mutant of Ustilago maydis was studied. Enzymatically inverted clarified juice (medium J-3) gave 2.9 g of lysine per liter under the following conditions: inoculum, 5%; pH 5.8; temperature, 30 C; K(La) in the fermentors, 0.41 mmoles of O(2) per liter per min; fermentation time, 72 hr. The concentrate, obtained by direct evaporation and drying of the fermentation broth, could be used as a possible feed supplement because of its amino-acid and vitamin content.
研究了利用先前获得的玉米黑粉菌突变体，在含有蔗糖、黑糖蜜或澄清甘蔗汁的培养基中外源生产L-赖氨酸的情况。在以下条件下，酶解转化的澄清汁（培养基J-3）每升可产生2.9克赖氨酸：接种量5%；pH值5.8；温度30℃；发酵罐中的K(La)为每分钟每升0.41毫摩尔氧气；发酵时间72小时。通过直接蒸发和干燥发酵液得到的浓缩物，因其氨基酸和维生素含量，可用作一种可能的饲料添加剂。
