Quesada-Chanto A, Afschar A S, Wagner F
GBF-Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany.
Appl Microbiol Biotechnol. 1994 Jun;41(4):378-83. doi: 10.1007/BF00939023.
With a cell concentration of 125 g dry biomass l-1 and a dilution rate of 0.1 h-1, Propionibacterium acidipropionici produces 30 g propionic acid l-1 from sugar with a productivity of 3 g l-1 h-1. The yield of propionic acid is approx. 0.36-0.45 g propionic acid g-1 sucrose and is independent of the dilution rate and cell concentration. Acetic acid is an unwanted by-product in the production of propionic acid. The concentration of acetic acid only increases slightly when the cell concentration is increased. A two-stage fermentation process was developed for the conversion of sugar or molasses of various types to propionic acid and vitamin B12. By fermentation of blackstrap molasses (from sugar beet and sugar cane) in the first fermentation stage 17.7 g propionic acid l-1 with a yield of 0.5 g propionic acid g-1 carbohydrate was produced with a dilution rate of 0.25 h-1. In the second stage 49 mg vitamin B12 1-1 was produced at a dilution rate of 0.03 h-1.
在细胞浓度为125克干生物量/升且稀释率为0.1小时-1的条件下，丙酸丙酸杆菌从糖中生产出30克/升的丙酸，生产速率为3克/升·小时。丙酸产量约为0.36 - 0.45克丙酸/克蔗糖，且与稀释率和细胞浓度无关。乙酸是丙酸生产中不需要的副产物。当细胞浓度增加时，乙酸浓度仅略有增加。开发了一种两阶段发酵工艺，用于将各种类型的糖或糖蜜转化为丙酸和维生素B12。在第一发酵阶段，通过发酵黑糖蜜（来自甜菜和甘蔗），在稀释率为0.25小时-1的情况下，生产出了17.7克/升的丙酸，丙酸产量为0.5克丙酸/克碳水化合物。在第二阶段，以0.03小时-1的稀释率生产出了49毫克/升的维生素B12。
