Badmin J A, Kumar J L, Mann W C
J Chromatogr. 1979 Apr 21;172:319-25. doi: 10.1016/s0021-9673(00)90968-3.
A simple and rapid method for the determination of buflomedil hydrochloride [2',4',6'-trimethoxy-4-(pyrrolidinyl)butyrophenone hydrochloride] in pharmaceutical formulations by reversed-phase ion-pair high-performance liquid chromatography is described. The sample is dissolved in methanol and 1,3,5-trimethoxybenzene is added as an internal standard. The resulting solution is chromatographed on an octadecylsilane column using ion-pair partition chromatography with lauryl sulphate as the counter ion. A 25-microliter injection (containing 3.5 micrograms of buflomedil hydrochloride) produces a 60% full-scale peak (0.2 a.u.f.s.) at the absorbance maximum (275 nm). The relative standard deviation of the method ranges from 1 to 3%, depending on the particular tablet formulation examined. Excipients present in the preparations do not interfere.
描述了一种采用反相离子对高效液相色谱法测定药物制剂中盐酸丁咯地尔[2',4',6'-三甲氧基-4-(吡咯烷基)丁酰苯盐酸盐]的简单快速方法。将样品溶解于甲醇中，并加入1,3,5-三甲氧基苯作为内标。所得溶液在十八烷基硅烷柱上进行色谱分析，采用以月桂基硫酸盐为反离子的离子对分配色谱法。进样25微升（含3.5微克盐酸丁咯地尔）在最大吸收波长（275纳米）处产生一个满量程60%的峰（0.2吸光度单位满量程）。该方法的相对标准偏差范围为1%至3%，具体取决于所检测的特定片剂剂型。制剂中存在的辅料不产生干扰。
