Chan K K, Wong C D
J Chromatogr. 1979 Apr 21;172:343-9. doi: 10.1016/s0021-9673(00)90971-3.
A rapid, non-hydrolytic thin-film fluorescence scanning method is described for the quantitation of adriamycin and metabolites in tissues. Adriamycin, with daunomycin added as the internal standard, was extracted from tissue homogenate which contained 500 micrograms of oxalic acid, with ice-cold 0.5 N hydrochloric acid--85% isopropanol, separated by thin-layer chromatography, and quantitated in situ via a fluorescence scanning technique. This method has a sensitivity to 0.05 microgram per gram of tissue.
本文描述了一种快速、非水解性的薄膜荧光扫描法，用于定量组织中的阿霉素及其代谢产物。以柔红霉素作为内标，从含有500微克草酸的组织匀浆中，用冰冷的0.5N盐酸-85%异丙醇提取阿霉素，通过薄层色谱法分离，并采用荧光扫描技术进行原位定量。该方法对每克组织中0.05微克的阿霉素具有检测灵敏度。
