Vilaginès R, McAuslan B R
J Virol. 1970 Sep;6(3):303-9. doi: 10.1128/JVI.6.3.303-309.1970.
Synthesis and maturation of frog virus 3 deoxyribonucleic acid (DNA) in BHK cells and selected variants, in chick fibroblasts, and in minnow cells were compared. Wide ranges in rates of DNA synthesis and assembly of virions were found. At least three variants of BHK cells can be obtained: (i) fully permissive, characterized by rapid DNA synthesis and assembly to give a high yield of infective virus; (ii) semipermissive, in which viral DNA is synthesized slowly for extended periods of time, and the yield of infective virus is poor; and (iii) nonpermissive, in which virus adsorbs to cells and arrests host functions but viral DNA is not synthesized. Kinetics of appearance of virions and procedures for their separation from cell extracts are described.
对蛙病毒3脱氧核糖核酸（DNA）在BHK细胞及其选定变体、鸡成纤维细胞和鲦鱼细胞中的合成与成熟进行了比较。发现病毒DNA合成速率和病毒粒子组装速率存在很大差异。可获得至少三种BHK细胞变体：（i）完全允许型，其特征是DNA快速合成并组装，产生高产量的感染性病毒；（ii）半允许型，其中病毒DNA长时间缓慢合成，感染性病毒产量低；（iii）非允许型，其中病毒吸附到细胞上并阻断宿主功能，但不合成病毒DNA。描述了病毒粒子出现的动力学及其从细胞提取物中分离的方法。
