Basilico C, Burstin S J
J Virol. 1971 Jun;7(6):802-12. doi: 10.1128/JVI.7.6.802-812.1971.
The multiplication of polyoma virus in a mouse-hamster (3T3 x BHK) somatic hybrid line (10A), which, although permissive for viral multiplication, produces very low amounts of virus, has been studied. In this cell line, the efficiency of productive infection is high, but the yield of infectious virus is on the order of 0.5% of that of 3T3 cells. The amount of viral deoxyribonucleic acid (DNA) synthesized by these cells upon infection is about 5% of that of 3T3 cells. An examination of the virus produced in hybrid 10A revealed that it was only one-tenth as infectious as the virus grown in 3T3. Although the viral DNA synthesized in the infected 10A cells is normal, the DNA extracted from purified virus grown in 10A consists of approximately 10% of normal, supercoiled polyoma DNA molecules and of approximately 90% linear DNA molecules with a sedimentation coefficient of 14 to 16S. These DNA molecules appear to be of cellular origin but contain a limited amount of viral DNA sequences. The host DNA-containing particles are not infectious but appear to possess some biological activity; they give rise to a weak complementation effect, and part of them are able to induce T-antigen synthesis. In addition, the host DNA present in these particles is predominantly that which has been synthesized after infection. The correlation between the block in viral DNA synthesis in this cell line and the abnormal encapsidation of host DNA is discussed.
对多瘤病毒在小鼠 - 仓鼠（3T3×BHK）体细胞杂交系（10A）中的增殖情况进行了研究，该杂交系虽允许病毒增殖，但产生的病毒量极少。在这个细胞系中，生产性感染的效率很高，但感染性病毒的产量约为3T3细胞产量的0.5%。这些细胞感染后合成的病毒脱氧核糖核酸（DNA）量约为3T3细胞的5%。对杂交系10A产生的病毒进行检查发现，其感染性仅为在3T3中生长的病毒的十分之一。尽管在感染的10A细胞中合成的病毒DNA是正常的，但从在10A中生长的纯化病毒中提取的DNA约10%由正常的超螺旋多瘤DNA分子组成，约90%是沉降系数为14至16S的线性DNA分子。这些DNA分子似乎起源于细胞，但含有有限数量的病毒DNA序列。含有宿主DNA的颗粒没有感染性，但似乎具有一些生物学活性；它们产生微弱的互补效应，其中一部分能够诱导T抗原合成。此外，这些颗粒中存在的宿主DNA主要是感染后合成的。本文讨论了该细胞系中病毒DNA合成受阻与宿主DNA异常包装之间的相关性。
