Rothen A
Physiol Chem Phys. 1979;11(6):481-9.
Tryptic action occurs when a layer of bovine serum albumin adsorbed on a nickel-plated slide and protected by a Formvar membrane 120 A thick is treated with dilute trypsin solution. But experimental evidence indicates that the trypsin molecules to not cross the membrane. Thus the proposal that trypsin can exert its enzymatic action without intimate contact with the substrate, first set forth in 1948 but later abandoned in favor of a "forced diffusion" hypothesis, now appears the correct interpretation. Analogously, antibodies can be specifically immobilized on one side of a membrane separating them from adsorbed antigens located on the other side.
当吸附在镀镍载玻片上并由厚度为120埃的福尔马膜保护的一层牛血清白蛋白用稀胰蛋白酶溶液处理时，就会发生胰蛋白酶作用。但实验证据表明，胰蛋白酶分子不会穿过该膜。因此，1948年首次提出但后来被放弃而支持“强制扩散”假说的关于胰蛋白酶可以在不与底物密切接触的情况下发挥其酶促作用的提议，现在看来是正确的解释。类似地，抗体可以特异性地固定在将它们与位于另一侧的吸附抗原隔开的膜的一侧。
