Shi Wei, Zhang Fengbao, Zhang Guoliang
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 May 25;819(2):301-6. doi: 10.1016/j.jchromb.2005.02.018.
Microporous polyamide membranes were activated by bisoxirane and subsequently bound with chitosan (CS) to amplify reactive groups. Then polylysine (PLL) as ligand was immobilized onto the CS-coated nylon membranes. The contents of CS and PLL of PLL-attached membranes were 93.2 and 90.4 mg/g nylon membrane, respectively. Such PLL-attached membranes were used to adsorb bilirubin from the bilirubin-phosphate solution and bilirubin-albumin solution. The adsorption mechanism of bilirubin and the effects of temperature, initial concentration of bilirubin, albumin concentration and ionic strength on adsorption were investigated by batch experiments. The results showed that the adsorption capacity increased with increasing the temperature while decreased with increasing the NaCl concentration and albumin concentration, and the adsorption isotherm fitted the Freundlich model well. The result of dynamic experiment showed PLL-attached membranes can well remove the bilirubin from the bilirubin-albumin solution.
用双环氧乙烷对微孔聚酰胺膜进行活化，随后与壳聚糖（CS）结合以增加反应基团。然后将作为配体的聚赖氨酸（PLL）固定在涂有CS的尼龙膜上。附着PLL的膜中CS和PLL的含量分别为93.2和90.4 mg/g尼龙膜。这种附着PLL的膜用于从胆红素 - 磷酸盐溶液和胆红素 - 白蛋白溶液中吸附胆红素。通过分批实验研究了胆红素的吸附机理以及温度、胆红素初始浓度、白蛋白浓度和离子强度对吸附的影响。结果表明，吸附容量随温度升高而增加，随NaCl浓度和白蛋白浓度升高而降低，吸附等温线与Freundlich模型拟合良好。动态实验结果表明，附着PLL的膜能够很好地从胆红素 - 白蛋白溶液中去除胆红素。
