Investigations on the biological activity of Tylopilus felleus (Bull. Ex Fr.) P. Karst. by means of phytobiological tests.

The effect of the undialysed (ND) aqueous fraction prepared from the fruitbodies of Tylopilus felleus and of actinomycin C on the biosynthesis of DNA in the seedling roots of Triticum vulgare and on the increase of their mass was investigated. The content of DNA (expressed in mug) in the determined amount of the seedling roots of Triticum vulgare after treating of material with the ND fraction, was 9-1--23-3% higher than in the control. The investigated fraction inhibited the increase of the mass of seedling roots in the range 24-5--48-0%. It seems possible that ND fraction inhibits the biosynthesis of the other components of the cells, while the synthesis rate of DNA is only slightly changed. ND fraction and N-hydroxyurea inhibited the linear growth and the increase of the mass of Lepidium sativum roots in the test acc. to Gagiu et al. [5]. Using the Constantinescu et al. test[3,4] the CMA value of polysaccharide fractions was calculated. The pycnose phenomenon in the course of cytological analysis of the meristematic cells of the wheat root seedlings treated with polysaccharide fraction was observed.