Bjornson A B, Bjornson H S
Rev Infect Dis. 1979 Mar-Apr;1(2):347-56. doi: 10.1093/clinids/1.2.347.
Studies were conducted to determine the requirements for immunoglobulin and complement in opsonization of Bacteroides fragilis and Bacteroides thetaiotaomicron. The ability of human sera depleted of immunoglobulin or of components of complement to promote the phagocytosis and intracellular killing of the two strains of Bacteroides by human leukocytes was measured in vitro under anaerobic conditions. Neither hypogammaglobulinemic sera nor pooled normal human serum that was heated at 56 C for 30 min supported phagocytosis and killing of the two strains of Bacteroides. Neither sera depleted of terminal complement components by treatment with inulin or cobra venom factor nor human serum deficient in C8 supported phagocytosis of the tested strains. In addition, pooled normal human serum depleted of C3, factor B, or factor D did not support phagocytosis of either strain. Dose-dependent restoration of the opsonic activity of factor B-depleted serum was accomplished by purified human factor B but not by human C2. The results indicate that immunoglobulin and components of the alternative comed in this study.
开展了多项研究以确定免疫球蛋白和补体在脆弱拟杆菌和多形拟杆菌调理吞噬作用中的需求。在厌氧条件下于体外测定了缺乏免疫球蛋白或补体成分的人血清促进人白细胞对这两种拟杆菌菌株进行吞噬和细胞内杀伤的能力。低丙种球蛋白血症血清以及在56℃加热30分钟的混合正常人血清均不支持对这两种拟杆菌菌株的吞噬和杀伤。用菊粉或眼镜蛇毒因子处理而缺乏末端补体成分的血清以及缺乏C8的人血清均不支持对受试菌株的吞噬作用。此外，缺乏C3、B因子或D因子的混合正常人血清也不支持对任何一种菌株的吞噬作用。用纯化的人B因子而非人C2可实现剂量依赖性恢复缺乏B因子血清的调理活性。结果表明，免疫球蛋白和替代途径的补体成分参与了本研究中的调理吞噬作用。 （注：原文最后一句“alternative comed”表述有误，可能是“alternative complement”，译文按推测修正）
