Greiff D
Appl Microbiol. 1970 Dec;20(6):935-8. doi: 10.1128/am.20.6.935-938.1970.
Suspensions of influenza virus were dried by sublimation of ice in vacuo to contents of residual moisture of 2.8, 1.6, or 0.6%. After drying, the preparations were sealed under helium, argon, nitrogen, or a mixture of oxygen and nitrogen (30% O(2), 70% N(2)). Stabilities of the several preparations were determined by an accelerated storage test. Based on the times predicted for the dried preparations stored at preselected temperatures to lose 1 log of infectivity titer, the order of stabilities in relation to residual moisture, gas was as follows: 1.6%, He > 0.6%, He > 2.8%, He > 1.6%, Ar > 2.8%, N(2) > 2.8%, Ar > 1.6%, N(2) > 2.8%, O(2) > 0.6%, Ar > 0.6%, N(2) > 1.6%, O(2) > 0.6%, O(2). The stability of the preparation sealed under helium and dried to the content of residual moisture found best for this gas (1.6%) resulted in an increased stability of the order of years as compared to the other preparations tested.
流感病毒悬液通过在真空中升华冰进行干燥，使残留水分含量达到2.8%、1.6%或0.6%。干燥后，制剂在氦气、氩气、氮气或氧气与氮气的混合物（30% O₂，70% N₂）下密封。通过加速储存试验测定几种制剂的稳定性。根据在预选温度下储存的干燥制剂失去1个对数感染性滴度所需的时间，与残留水分、气体相关的稳定性顺序如下：1.6%，氦气＞0.6%，氦气＞2.8%，氦气＞1.6%，氩气＞2.8%，氮气＞2.8%，氩气＞1.6%，氮气＞2.8%，氧气＞0.6%，氩气＞0.6%，氮气＞1.6%，氧气＞0.6%，氧气。在氦气下密封并干燥至该气体最适宜的残留水分含量（1.6%）的制剂稳定性，与其他测试制剂相比，稳定性提高了数年。
