性激素对大鼠肝细胞溶胶中2-（N-羟基-乙酰氨基）芴酶促酯化作用的影响。

Enzymic esterifications of 2-(N-hydroxyacetamido)fluorene and several other hydroxamic acids by liver cytosol were studied. Determination of 2-acetamido-3-methylthiofluorene was used for the assay. 2. With rat liver cytosol, requirement for ATP, Mg(2+) and SO(4) (2-) suggested formation of phosphate and sulphate esters of 2-(N-hydroxyacetamido)fluorene. 3. Rats showed sex and age differences in their activity. Liver from adult male rat was at least twice as active as liver from adult female rat. No such sex differences were found in mice, hamsters and guinea pigs. 4. Administration of testosterone (300mug/day) subcutaneously for 8 days increased the activity in the female rat by 100%, whereas diethylstilboestrol (100mug/day) had no effect. In the male rat diethylstilboestrol treatment decreased the activity by 60%, whereas testosterone pretreatment was without any effect. 5. Among various endocrine ablations such as adrenalectomy, castration, adrenalectomy-castration and hypophysectomy in the adult male rat, hypophysectomy was found to be the most effective in decreasing the activity of the liver to about 50% of control values. 6. Like 2-(N-hydroxyacetamido)fluorene, various other N-hydroxy derivatives of 2-acetamido-7-fluorofluorene, 2-acetamidophenanthrene, 4-acetamidobiphenyl and 4-acetamidostilbene were also shown to be esterified to different extents by rat liver cytosol.

研究了肝细胞质溶胶对2-(N-羟基乙酰氨基)芴及其他几种异羟肟酸的酶促酯化作用。采用测定2-乙酰氨基-3-甲硫基芴的方法进行检测。
对于大鼠肝细胞质溶胶，对ATP、Mg(2+)和SO(4)(2-)的需求表明形成了2-(N-羟基乙酰氨基)芴的磷酸酯和硫酸酯。
大鼠在活性方面表现出性别和年龄差异。成年雄性大鼠的肝脏活性至少是成年雌性大鼠肝脏活性的两倍。在小鼠、仓鼠和豚鼠中未发现这种性别差异。
皮下注射睾酮（300μg/天）8天可使雌性大鼠的活性增加100%，而己烯雌酚（100μg/天）则无作用。在雄性大鼠中，己烯雌酚处理可使活性降低60%，而睾酮预处理则无任何作用。
在成年雄性大鼠进行的各种内分泌切除手术中，如肾上腺切除术、去势术、肾上腺切除-去势术和垂体切除术，发现垂体切除术对降低肝脏活性最为有效，可降至对照值的约50%。
与2-(N-羟基乙酰氨基)芴一样，2-乙酰氨基-7-氟芴、2-乙酰氨基菲、4-乙酰氨基联苯和4-乙酰氨基芪的各种其他N-羟基衍生物也被证明可被大鼠肝细胞质溶胶不同程度地酯化。

Effects of sex hormones on enzymic esterification of 2-(N-hydroxy-acetamido) fluorene by rat liver cytosol.