通过沉淀法浓缩裂谷热病毒和基孔肯雅病毒。
Concentration of Rift Valley fever and Chikungunya viruses by precipitation.
作者信息
Klein F, Mahlandt B G, Cockey R R, Lincoln R E
出版信息
Appl Microbiol. 1970 Sep;20(3):346-50. doi: 10.1128/am.20.3.346-350.1970.
Simple and efficient methods for concentrating Rift Valley fever (RVF) virus and chikungunya (CHIK) virus are described. Ammonium sulfate, potassium sulfate, or alcohol was used as a precipitating agent and the precipitate was resuspended to volumes suitable for further processing and purification. The methods permitted concentration of live RVF virus and CHIK virus about 100-fold with negligible losses of virus. RVF virus retained a high level of infectivity with potassium aluminum sulfate and alcohol, but CHIK virus retained a higher infectivity level with ammonium sulfate than with potassium aluminum sulfate. The data indicate that serum plays an important role in the concentration of both viruses, at least when the sulfate methods are used.
本文描述了用于浓缩裂谷热（RVF）病毒和基孔肯雅热（CHIK）病毒的简单高效方法。使用硫酸铵、硫酸钾或酒精作为沉淀剂，将沉淀物重悬至适合进一步处理和纯化的体积。这些方法可将活的RVF病毒和CHIK病毒浓缩约100倍，病毒损失可忽略不计。RVF病毒用硫酸铝钾和酒精处理后仍保持高感染性，但CHIK病毒用硫酸铵处理后的感染性水平高于用硫酸铝钾处理后的水平。数据表明，血清在两种病毒的浓缩过程中起着重要作用，至少在使用硫酸盐方法时如此。
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