Matsubara K, Aoki T, Kawashima S, Saito R, Ogura Y
Auris Nasus Larynx. 1979;6(1):1-11. doi: 10.1016/s0385-8146(79)80002-4.
This paper shows a casting method of the vascular system of the inner ear of the guinea pig with the use of fluid resin injected intravascularly and the observation of those specimens by means of the scanning electron microscopy. First, fluid resin was injected into the blood vessels of a live or just sacrificed guinea pig. After taking the temporal bone out of the animal, it was decalcified and macerated completely. Then, a cast of the inner ear vessels was taken and observed with a scanning electron microscope. A pair of photos differing in teh view angle by seven degrees were taken simultaneously, and observed with a stereoviewer. This technique brought photographs of well magnified three-dimensional construction of the inner ear blood vessels.
本文展示了一种通过向豚鼠内耳血管内注射液体树脂来铸造其血管系统的方法，并借助扫描电子显微镜对这些标本进行观察。首先，将液体树脂注入活的或刚处死的豚鼠的血管中。从动物体内取出颞骨后，对其进行脱钙并完全浸软。然后，获取内耳血管的铸型并用扫描电子显微镜进行观察。同时拍摄一对视角相差7度的照片，并用立体观察器进行观察。这项技术带来了放大良好的内耳血管三维结构的照片。
