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[利用扫描电子显微镜对豚鼠柯蒂氏器进行伪三维观察]

[Pseudo-three dimensional observation of the organ of Corti in the guinea pig using scanning electron microscopy].

作者信息

Rogowski M, Reiss G

机构信息

Z Kliniki Otolaryngologii Akademii Medycznej w Białymstoku.

出版信息

Otolaryngol Pol. 1994;48(4):391-7.

PMID:7970787
Abstract

The entire organ of Corti of guinea pig was investigated by scanning electron microscopy (SEM), using it's high resolving power to observe the three dimensional fine structure of the cochlea. Inner ear tissue of the normal guinea pig was conductively stained (OTOTO-method) for SEM. The relationship between the sensory cells and supporting cells was shown. SEM gives a nice overview of the surface structure of three dimensional organs like the inner ear. We conclude that bulk specimens of the organ of Corti are more advantageous than piece specimens in acoustic physiological as well as pathological research studies.

摘要

利用扫描电子显微镜(SEM)的高分辨率观察豚鼠整个柯蒂氏器的三维精细结构,对其进行了研究。采用导电染色法(OTOTO法)对正常豚鼠的内耳组织进行SEM观察,展示了感觉细胞与支持细胞之间的关系。SEM能很好地呈现内耳等三维器官的表面结构。我们得出结论,在听觉生理和病理研究中,柯蒂氏器的整块标本比切片标本更具优势。

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