表皮透明角质颗粒的化学性质。
The chemical nature of keratohyalin granules of the epidermis.
作者信息
Matoltsy A G, Matoltsy M N
出版信息
J Cell Biol. 1970 Dec;47(3):593-603. doi: 10.1083/jcb.47.3.593.
Abstract
Keratohyalin granules were isolated in the native form from the epidermis of newborn rats by the use of citric acid and a detergent. The isolated granules revealed a fine granular substructure in the electron microscope similar to that seen in situ. Analyses of amino acids by automated column-chromatography showed that proline and cystine are present in large proportions whereas histidine is present in a small amount. Accordingly, it was concluded that keratohyalin represents a sulfur-rich amorphous precursor of the horny cell content, rather than a sulfur-poor side product of the keratinization process, or a unique histidine-rich protein as proposed by in situ histochemical and radioautographic studies.
摘要
通过使用柠檬酸和一种去污剂，从新生大鼠的表皮中以天然形式分离出透明角质颗粒。在电子显微镜下，分离出的颗粒呈现出精细的颗粒亚结构，与原位观察到的相似。通过自动柱色谱法对氨基酸进行分析表明，脯氨酸和胱氨酸含量很高，而组氨酸含量很少。因此，得出结论：透明角质是角质形成细胞内容物中富含硫的无定形前体，而不是角质化过程中含硫量低的副产物，也不是原位组织化学和放射自显影研究中所提出的富含组氨酸的独特蛋白质。
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本文引用的文献
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The membrane protein of horny cells.角质形成细胞的膜蛋白。
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