梨形四膜虫细胞核结构与功能的研究。II. 大核和小核的分离。
Studies on nuclear structure and function in Tetrahymena pyriformis. II. Isolation of macro- and micronuclei.
作者信息
Gorovsky M A
出版信息
J Cell Biol. 1970 Dec;47(3):619-30. doi: 10.1083/jcb.47.3.619.
Abstract
This report describes a rapid, efficient method for isolating macronuclei from Tetrahymena. The macronuclear fraction contains only small amounts of micronuclear material and little detectable whole cell or cytoplasmic contamination. A method is also described for preparing a "micronuclear fraction" which contains 20-40 micronuclei for every macronucleus present. Electron microscope observations indicate that the ultrastructure of the nuclei in the macronuclear fraction closely resembles that of nuclei in situ. The presence of ribosomes on the outer membrane of micronuclei and of pores in the micronuclear envelope is also described.
摘要
本报告描述了一种从四膜虫中分离大核的快速、高效方法。大核组分仅含有少量的小核物质，几乎检测不到全细胞或细胞质污染。还描述了一种制备“小核组分”的方法，该组分中每一个大核含有20 - 40个小核。电子显微镜观察表明，大核组分中细胞核的超微结构与原位细胞核的超微结构非常相似。还描述了小核外膜上核糖体的存在以及小核膜孔的情况。
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本文引用的文献
1
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J R Microsc Soc. 1964 Jun;83:161-7. doi: 10.1111/j.1365-2818.1964.tb00525.x.
2
STUDIES ON ISOLATED NUCLEI. I. ISOLATION AND CHEMICAL CHARACTERIZATION OF A NUCLEAR FRACTION FROM GUINEA PIG LIVER.豚鼠肝脏细胞核的研究。I. 豚鼠肝脏细胞核组分的分离与化学特性分析
J Cell Biol. 1963 Aug;18(2):267-91. doi: 10.1083/jcb.18.2.267.
3
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.在电子显微镜检查中，将高pH值的柠檬酸铅用作电子不透明染色剂。
J Cell Biol. 1963 Apr;17(1):208-12. doi: 10.1083/jcb.17.1.208.
4
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J Cell Biol. 1962 Mar;12(3):515-31. doi: 10.1083/jcb.12.3.515.
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Improvements in epoxy resin embedding methods.环氧树脂包埋方法的改进。
J Biophys Biochem Cytol. 1961 Feb;9(2):409-14. doi: 10.1083/jcb.9.2.409.
6
[Spectrophotometric determination of total nucleic acids].[分光光度法测定总核酸]
Biokhimiia. 1958;23(5):656-62.
7
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9
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Nature. 1966 Feb 5;209(5023):630-1. doi: 10.1038/209630b0.
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