使用¹⁵N 代谢标记定量组蛋白修饰。
Quantification of histone modifications using ¹⁵N metabolic labeling.
机构信息
Department of Computational Medicine and Bioinformatics, University of Michigan, USA.
出版信息
Methods. 2013 Jun 15;61(3):236-43. doi: 10.1016/j.ymeth.2013.02.004. Epub 2013 Feb 27.
Abstract
Mass spectrometry has made major contributions to recent discoveries in the field of epigenetics, particularly in the characterization of the myriad post-translational modifications (PTMs) of histones which are technically challenging to analyze. These new developments have further aroused great interest in development of robust, new mass spectrometric methods to quantitatively study the dynamics of histone modifications. This review covers quantitative analysis of histone PTMs and discuss an ¹⁵N metabolic labeling procedure for quantifying histone PTMs applied to the analysis of methyltransferase knockouts in the model organism, Tetrahymena thermophila.
摘要
质谱分析在表观遗传学领域的最新发现中做出了重大贡献，特别是在鉴定技术上具有挑战性的组蛋白的多种翻译后修饰（PTMs）方面。这些新的发展进一步激发了人们对开发强大的新质谱方法的极大兴趣，以便定量研究组蛋白修饰的动力学。本综述涵盖了组蛋白 PTM 的定量分析，并讨论了一种¹⁵N 代谢标记程序，用于定量分析组蛋白 PTM，该程序应用于模式生物嗜热四膜虫中转录酶缺失的分析。
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